猪链球菌2型的纤连蛋白/血纤维蛋白原结合蛋白基因的序列分析

孙理云; 范红结; 陆承平

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微生物与感染 ›› 2006, Vol. 1 ›› Issue (1) : 28-31.

论著

猪链球菌2型的纤连蛋白/血纤维蛋白原结合蛋白基因的序列分析

孙理云; 范红结; 陆承平

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Cloning and sequence analysis of fbps gene of Streptococcus suis type2

SUN Li-yun; FAN Hong-jie; LU Cheng-ping

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摘要

目的为确定猪链球菌2型(SS2)江苏分离株9801是否具有纤连蛋白／血纤维蛋白原结合蛋白基因(fbps)。方法根据已发表的SS2 fbps序列,设计并合成1对引物,以SS2江苏分离株的基因组为模板,采用聚合酶链反应(PER)方法扩增fbps,克隆于pMD-T18载体。测定阳性质粒插入序列。利用DNAstar软件,比较所测序列与不同来源SS2的fbps和FBPS与链球菌属其他种同源蛋白的同源性。结果扩增的fbps为1 938 bp,江苏分离株与猪源致病性SS2荷兰分离株的fbps序列有5个碱基不同,与ATCCA3765株有3个碱基不同,同源性均达99．99％以上。推导的氨基酸序列比较,分别有4个和2个氨基酸不同,同源性均为99％以上。纤连蛋白结合蛋白(FBPS)无前导序列,无锚定序列,与链球菌属其他种的同源Fn结合蛋白的同源性为68．8％～76．0％。结论 FBPS是一种无锚的黏附素。

Abstract

Objective To define whether Streptococcus suis type 2 Jiangsu isolate HA9801 prassesses, fbps gene[encoding fibronectin-and fibrinogen-binding protein(FBPS)] and to study its sequence. Methods fbps gene was amplified from genomic DNA of Streptococcus suis type 2 Jiangsu isolate HA9801 by polymerase chain reaction (PCR). PCR product was cloned into the cloning vector pMDT-18. The recombinant fragment of 1 938bp was verified by restriction endonuclease analysis and sequencing. Results The fbps gene nucleotide sequence of Jiangsu isolate was more than 99.99% identical to that of SS2 Netherland strain as reported by Greeff and to that of ATCC strain. FBPS displayed 76.0%, 73.0%, 72.4%, 70.1% and 68.8% identity with FbpA of S. gordonii (CAA4628.2), putative fibronectin/ fibrinogen-binding protein of S. mutan (AAN59108.1), PavA of S. pneumoniae, (AAK75087.1), adherence and virulence protein A of S. agalactiae 2603V/R(AAN00072.1) and FBP54 of S. pyogenes(AAA57236),respectively. Conclusion Based on stucture analysis,FBPS was predicted as an adhesin without anchoring component.