Abstract：Objective To establish an antibody-sandwich enzyme linked immunosorbent assay (ELISA) for the detection of Ureaplasm urealyticum (UU) . Methods We used an antibody sandwich ELISA to detect UU and compared the results with cell culture method. Results The rate of sensitivity of the ELISA was 92.6% and the rate of specificity was 97.4% . The lowest detection limitation was 5～10 ng/ml of UU antigen. Conclusion The antibody-sandwich ELISA is a sensitive, convenient and fast method for detection of UU for the screening of large numbers of specimens.