Loss of Balance between T Helper Type 17/Regulatory T Cells in HIV Infected Chinese Patients
LI Dan; JIA Man-Xue; LIANG Hua; PENG Hong; LIU Dong-Hua; ZHAO Yang; SHAO Yi-Ming
State Key Laboratory for Infectious Disease Control and Prevention, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing102206, China
摘要: 通过探讨人类免疫缺陷病毒(HIV)感染者外周血中Th17与 CD4+CD25hiFoxp3+调节性T(Treg)细胞比例及Th17/Treg细胞平衡状态与疾病进展的关系,初步阐明Th17/Treg细胞失衡在HIV发病机制中的作用和意义。选取54例未经抗病毒治疗的HIV感染者,另有32名健康志愿者作为正常对照。分离外周血单核细胞后,利用流式细胞技术检测Th17和Treg细胞水平。结果表明,在HIV感染者外周血中Th17细胞比例明显低于正常对照组(0.68±0.35 vs 1.42±0.86,P<0.001),Treg细胞比例明显高于正常对照(6.15±2.12 vs 4.50±0.76,P<0.001), 导致HIV感染者中Th17/Treg细胞比例较正常对照显著降低,(0.12±0.07 vs 0.31±0.17,P<0.001)。研究还发现,Th17/CD4细胞比例与CD4+ T细胞计数正相关(r=0.371,P<0.05),与病毒载量不相关。Treg/CD4细胞比例与CD4 +T细胞计数负相关,与病毒载量正相关(r=-0.402,0.447;P<0.05,<0.001)。此外,Th17/Treg细胞比例与CD4+ T细胞计数正相关,与病毒载量负相关(r=0.525,-0.318;P<0.001,<0.05)。结果提示,HIV感染中存在Th17/Treg细胞比例失衡现象,与疾病进程密切相关,可能在HIV进展中具有重要作用。
Abstract:The aim of this study is to characterize the changes in interleukin 17 (IL-17)-secreting T helper type 17 (Th17) cell frequencies and CD4+CD25hiFoxP3+ regulatory T (Treg) in a cohort of chronic human immunodeficiency virus type 1 (HIV-1)-infected patients in China, and to clarify the role of Th17/Treg imbalance in HIV pathogenesis. A total of 54 untreated chronic HIV-infected individuals and 32 healthy donors were recruited in this study. Peripheral blood mononuclear cells were isolated from EDTA-anticoagulated fresh whole blood and stained to characterize Th17/CD4 and Treg/CD4. Our results indicated that the depletion of Th17 cells (0.68±0.35 vs 1.42±0.86, P<0.001) was accompanied by an increased Treg cells (6.15±2.12 vs 4.50±0.76, P<0.001), resulting in a loss of Th17/Treg balance in HIV infection (0.12±0.07 vs 0.31±0.17, P<0.001). Additionally, Th17/CD4 was positively correlated to CD4 T cell counts (r=0.371,P<0.05), Treg/CD4 was inversely related to CD4+ T cell counts and positively correlated to plasma viral load (r=-0.402, 0.447; P<0.05, <0.001). A positive correlation was observed between Th17/Treg with CD4+ T cell counts and Th17/Treg was negatively correlated to viral load (r=0.525, -0.318; P<0.001, <0.05). These results indicate that a loss of immune-balance during HIV-1 disease progression may have a critical role in HIV-1 infection and further shed new light into understanding the pathogenesis of HIV-1.
李丹; 贾曼雪; 梁华; 彭虹; 刘东华; 赵阳; 邵一鸣. 人类免疫缺陷病毒1型同性恋感染者与健康人群Th17/Treg平衡状况的比较研究[J]. 微生物与感染
, 2011, 6(2): 65-71.
LI Dan; JIA Man-Xue; LIANG Hua; PENG Hong; LIU Dong-Hua; ZHAO Yang; SHAO Yi-Ming. Loss of Balance between T Helper Type 17/Regulatory T Cells in HIV Infected Chinese Patients. Journal of Microbes and Infections, 2011, 6(2): 65-71.