Screening of standard clinic strains for animal model of drug-resistant Mycobacterium tuberculosis
DING Hai-Rong1, LIN Shu-Zhu1, LU Jin-Biao2, DENG Hai-Qing3, WANG Guo-Zhi2, QIN Chuan1, ZHAN Ling-Jun1
1. Institute of Laboratory Animal Science, Peking Union Medical College; Chinese Academy of Medical Science (CAMS) & Comparative Medicine Center; Key Laboratory of Human Disease Animal Model, State Administration of Traditional Chinese Medicine, Beijing 100021, China; 2. Division of Tuberculosis Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China; 3. Institute of Environmental Safety and Health Risks Evaluation, Wenzhou Medical University, Wenzhou 325035, China
Abstract：To screen out the standard clinic strains used for animal model of drug-resistant Mycobacterium tuberculosis (M. tuberculosis), the strains were collected from tuberculosis patients in several provinces of China. The drug susceptibility test with first-line and second-line anti-tuberculosis drugs and drug-resistant gene mutant analysis were performed in 98 clinic isolations, the strains with explicit drug resistant phenotype and mutant were selected for in vivo virulence experiment in mice. Among the 40 strains with explicit drug resistant patterns, the half lethal time (LT50) of 35 strains were shorter than that of H37Rv in the virulence experiment in mice. 18 in 35 strains were rifampin-resistant and isoniazid-resistant. 5 strains were rifampin-resistant, 7 strains were isoniazid-resistant, and 5 strains were sensitive to both isoniazid and rifampin. Standard strain with moderate virulence and explicite genotype was screened out for each drug resistant phenotype. For rifampin-resistance combined with isoniazid-resistance, one strain with LT50≤7 d was selected as its standard strong virulence. For rifampin-resistance or isoniazid-resistance, one strain with LT50≤7 d was selected as its standard strong virulence strain respectively. Each standard strain can be used as candidate standard strain used in M. tuberculosis infection model of guinea pig or other animal.
丁海榕1，林树柱1，卢锦标2，邓海清3，王国治2，秦川1，占玲俊1 . 耐药结核分枝杆菌动物模型所用感染菌株的筛选[J]. 微生物与感染, 2014, 9(2): 83-88.
DING Hai-Rong1, LIN Shu-Zhu1, LU Jin-Biao2, DENG Hai-Qing3, WANG Guo-Zhi2, QIN Chuan1, ZHAN Ling-Jun1. Screening of standard clinic strains for animal model of drug-resistant Mycobacterium tuberculosis. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(2): 83-88.
