微生物与感染
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微生物与感染
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发热伴血小板减少综合征病毒细胞表面受体的初步研究
张硕,李建东,曲靖,张福顺,李川,梁米芳,李德新
中国疾病预防控制中心病毒病预防控制所,国家卫生和计划生育委员会医学病毒学重点实验室,北京 102206
Study on cell surface receptors of severe fever with thrombocytopenia syndrome virus
ZHANG Shuo, LI Jian-Dong, QU Jing, ZHANG Fu-Shun, LI Chuan, LIANG Mi-Fang, LI De-Xin
Key Laboratory forMedical Virology, National Health and Family Planning Commission; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
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摘要: 为初步研究树突细胞特异性细胞间黏附分子-3结合非整合素因子(DC-SIGN)是否能作为严重发热伴血小板减少综合征病毒(SFTSV)的细胞表面受体,首先通过实时反转录-聚合酶链反应(RT-PCR)检测不同细胞系DC-SIGN mRNA水平及SFTSV感染水平,并用DC-SIGN特异性单克隆抗体封闭SFTSV敏感细胞,研究其对SFTSV感染水平的影响。此外,将DC-SIGN表达载体转染不同细胞系,研究转染前后细胞中SFTSV感染水平的变化。结果显示,在SFTSV感染水平较高的Vero细胞中,DC-SIGN mRNA水平较高;而在SFTSV感染水平低于Vero细胞的CHO-K1细胞中,DC-SIGN mRNA水平较低。DC-SIGN单克隆抗体在一定程度上能抑制病毒进入宿主细胞,并呈现剂量-效应关系,DC-SIGN可提高Vero和CHO-K1细胞中SFTSV的感染水平。本研究显示DC-SIGN为SFTSV的可能受体。

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张硕
李建东
曲靖
张福顺
李川
梁米芳
李德新
关键词 严重发热伴血小板减少综合征病毒树突细胞特异性细胞间黏附分子-3结合非整合素因子受体    
Abstract:To study cell surface receptors of severe fever with thrombocytopenia syndrome virus (SFTSV), the levels of dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) mRNA and SFTSV infection in different cell lines were identified by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). SFTSV-sensitive cells were inhibited by anti-DC-SIGN monoclonal antibody (mAb) and virus infection levels were detected by real-time RT-PCR. Different cell lines were transfected with DC-SIGN-expressing plasmid and SFTSV infection levels either before or after transfection were detected. The results showed that Vero cells which were sensitive to SFTSV had a relatively higher DC-SIGN level than CHO-K1 cells which were less sensitive to SFTSV. Anti-DC-SIGN mAb was capable to inhibit SFTSV invasion into host cells to a certain extent, showing a dose-response effect. The levels of SFTSV infection in cells transfected with DC-SIGN-expressing plasmid were higher than those in non-transfected cells. It is suggested that DC-SIGN is probable one of the cell surface receptors of SFTSV.

Key wordsSevere fever with thrombocytopenia syndrome virus    dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin    Receptor
通讯作者: 李德新   
引用本文:   
张硕,李建东,曲靖,张福顺,李川,梁米芳,李德新. 发热伴血小板减少综合征病毒细胞表面受体的初步研究[J]. 微生物与感染, 2014, 9(2): 112-115.
ZHANG Shuo, LI Jian-Dong, QU Jing, ZHANG Fu-Shun, LI Chuan, LIANG Mi-Fang, LI De-Xin. Study on cell surface receptors of severe fever with thrombocytopenia syndrome virus. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(2): 112-115.
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