Abstract:This study aims to isolate lytic bacteriophages from Enterococcus faecalis (E. faecalis),analyze its biological characteristics and to establish the research basis for a phagebased therapy against Enterococcus infections. A lytic bacteriophage (named φEn-ZZ8) was isolated from the sewage using doubleagar method and its basic biological characteristics were analyzed. The results showed that φEn-ZZ8 had an icosahedral head of 40 nm in diameter and a filamentous tail of 200 nm in length. The adsorption rate of φEn-ZZ8 reached 90% in 10 min. Onestep growth showed that φEn-ZZ8 had a short latency period of 15 min, a rise period of 40 min, and a burst size of 150 pfu/cell. The genome of φEn-ZZ8 was sensitive to HindIII, EcoRV, NdeI, PstI, Xba I and Sac II, and the size of the genome was estimated to be 42 kb. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) revealed that at least 14 proteins were observed on the gel, ranging from 11 000 to 130 000. The bacteriophage specifically infected and lysed 30.8% E. faecalis isolates. The φEn-ZZ8 also exhibited stability to the wide range of pH and temperature. The data suggest that phage φEn-ZZ8 may be a candidate therapeutic agent to control Enterococcus infections.
张哲,李菁华,逯茵茵,韩放,李诗恒,孙延波. 粪肠球菌裂解性噬菌体φEn-ZZ8生物学特性的初步分析[J]. 微生物与感染, 2015, 10(4): 241-246.
ZHANG Zhe, LI Jing-Hua, LU Yin-Yin, HAN Fang, LI Shi-Heng, SUN Yan-Bo. Biological characteristics of a virulent bacteriophage φEn-ZZ8 isolated from Enterococcus faecalis. JOURNAL OF MICROBES AND INFECTIONS, 2015, 10(4): 241-246.