Transcriptome analysis of clinically isolated human astrovirus in CaCo-2 cells
WU Li-Meng1,2,*, TENG Zheng1,*, LIU Jing2, ZHANG Xi1, CUI Xiaoxian2, LIN Qingneng1, WU Huanyu1, YUAN Zheng‘an1, XIE Youhua2
1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China; 2. Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
Abstract:Human astrovirus (HAstV) is one of the major causes of viral gastroenteritis in infants and young children. However, data on the host response to and pathogenesis of HAstV infection are limited. The primary goal of this study was to identify differentially expressed genes and enriched pathways associated with HAstV infection in human CaCo-2 cells. The clinical isolates of HAstV were used to infect CaCo-2 cells and total viral RNAs in the extracellular medium were quantitatively detected. Realtime reverse transcriptasepolymerase chain reaction (RT-PCR) assay was applied to clearly demonstrate a significant increase in the amount of viral RNAs in the culture supernatant at 9-24 h postinfection. Finally, RNA sequencing was performed to compare the transcriptomes of CaCo-2 cells infected with and without a clinically isolated HAstV strain. Statistical analyses on differentially expressed genes revealed that axon guidance and Ras signaling pathways were altered in HAstV5-infected CaCo-2 cells (P<0.05). This is the first report on the deep-sequencing analysis of transcripts of CaCo-2 cells infected with HAstV. The data from this study can serve as a basis for future studies of HAstV pathogenesis.
吴立梦1,2,*,滕峥1,*,刘晶2,张曦1,崔晓娴2,林庆能1,吴寰宇1,袁政安1,谢幼华2. 人星状病毒临床分离株感染CaCo-2细胞的转录组分析[J]. 微生物与感染, 2016, 11(2): 79-86.
WU Li-Meng1,2,*, TENG Zheng1,*, LIU Jing2, ZHANG Xi1, CUI Xiaoxian2, LIN Qingneng1, WU Huanyu1, YUAN Zheng‘an1, XIE Youhua2. Transcriptome analysis of clinically isolated human astrovirus in CaCo-2 cells. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(2): 79-86.