为研究上海长宁地区成人轮状病毒急性胃肠炎的分子特征，收集2010年6月—2013年12月长宁区定点医院成人急性胃肠炎患者的粪便标本1 554份，采用酶联免疫吸附试验(enzyme-linked immunosorbent assay，ELISA)进行A组轮状病毒检测，多重聚合酶链反应(polymerase chain reaction，PCR)进行B组和C组轮状病毒检测。结果显示，1 554例患者的平均年龄为(46.19±15.59)岁，其中男性691例、女性863例。共检出轮状病毒200株，检出率为12.87%。18～39岁组的轮状病毒检出率为9.15%，40～59岁组为15.58%，60～79岁组为15.16%，≥80岁组为18.57%。A组轮状病毒基因型检测结果显示，G型以G9亚型最多(29.82%)，P型以P[8]亚型最多(77.19%)，GP组合以G9P[8]最多(25.73%)。结果提示，2010—2013年上海长宁地区成人轮状病毒急性胃肠炎在就诊患者中以中老年多见，基因型以G9P[8]最多。

In order to study the molecular characteristics of rotavirus infection in adults with acute gastroenteritis in Shanghai, 2010-2013, the stool specimens collected in the period of June 2010 to December 2013 from 1 554 adult patients with acute gastroenteritis in Changning District were subjected to enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) for rotavirus detection. The results showed that the average age of all patients was (46.19±15.59) years old. Among them, 691 were male and 863 were female. Rotavirus was detected in 200 cases, with a detection rate of 12.87%. The detection rate of rotavirus was 9.15% in the patients aged 18-39, 15.58% in those aged 40-59, 15.16% in those aged 60-79, and 18.57% in those aged ≥80. The G9 subtype (29.82%) and P8 subtype (77.19%) were the most common genotypes in group A rotavirus, and G9P[8] was the most common GP combination. The results suggest that rotavirus infection in adults with acute gastroenteritis is more popular in middle aged and elderly people.