Abstract：Ureaplasma urealyticum (U. urealyticum) is a very important pathogenic microorganism. In view of a worsen resistance of U. urealyticum to antibiotics recently, it’s in great need to exploit new alternative therapies for U. urealyticum infections. The aim of this research is to study the in vitro susceptibility of U. urealyticum to toluidine blue-mediated photodynamic antimicrobial chemotherapy (PACT). Two biovars of U. urealyticum including two standard strains and two clinical strains were incubated with photosensitizer toluidine blue with different concentrations from 2.5 to 0.039 062 5 mmol/L. After 20 or 60 min incubation, they were treated with (633±10) nm red light source, with four different light fluences including 48, 102, 204 and 408 mJ/cm2. The association between the incubation time, toluidine blue concentration, as well as the light fluence and PACT effect on U. urealyticum was evaluated 48 h after treatment. Besides, the difference in response to PACT between the two biovars of U. urealyticum was also analyzed. The results showed that PACT had an inactivation effect on the growth of U. urealyticum, in a toluidine blue concentration-dependent and fluence-dependent manner. Longer incubation time induced more extensive inactivation of U. urealyticum. No significant difference in susceptibility to PACT was observed between the two biovars of U. urealyticum. It is concluded that toluidine blue-mediated PACT can inactivate U. urealyticumin vitro, and this therapy may become a promising alternative treatment for U. urealyticum infections.
叶庭路1，陈办成1，于波1，杨虹2，姜彬3，邵勇1，黄国新1. 光动力抗微生物化学疗法对解脲脲原体体外活性的影响[J]. 微生物与感染, 2018, 13(1): 16-20.
YE Tinglu1, CHEN Bancheng1, YU Bo1, YANG Hong2，JIANG Bin3，SHAO Yong1，HUANG Guoxin1. Effects of photodynamic antimicrobial chemotherapy on in vitro activity of Ureaplasma urealyticum. JOURNAL OF MICROBES AND INFECTIONS, 2018, 13(1): 16-20.
2018, Vol. 13 
