本研究针对10种对人类健康威胁较大的烈性病毒建立反转录-环介导等温扩增法(reverse transcription-loop-mediated isothermal amplification，RT-LAMP)，筛选出10种烈性病毒高特异性引物，并分别对这10种病毒进行特异检测。结果显示，其安全、便捷、快速，且高通量。在自主研发的RT-LAMP体系中，只需加入模板，结合配套仪器，扩增耗时不超过45 min，检测灵敏度为1～100拷贝/反应(25 μL)。检测30份人血清样品，筛查出1份裂谷热病毒阳性。该方法操作简单，可防止污染，成本低，易携带，方便于低配实验室或现场进行实时高通量检测。结果提示，针对10种烈性病毒的RT-LAMP方法可对口岸等人员流动性大的地区疑似患者进行快速筛查，展现出良好的应用前景。

The present study aims to establish a method for rapid detection of a panel of pathogenic viruses based on reverse transcription-loop-mediated isothermal amplification (RT-LAMP). Primer sets of 10 pathogenic viruses with high specificity were screened. It was suggested that the method was safe, rapid, convenient, and high-throughput in detection of pathogenic viruses. When combined with the new immobilized reaction tubes, the amplification for 10 pathogenic viruses in 1-100 copies could be finished within 45 min. In the assay of 30 serum samples, one rift valley fever virus-positive sample was obtained. A rapid, convenient, low-cost, real-time and high-throughput detection method for common pathogenic viruses was established. The results indicate that RT-LAMP method for 10 high-pathogenic viruses has a good application prospect in rapidly screening suspected patients in areas with high population mobility such as ports.