Abstract:Enterovirus type 71 (EV71) infection usually causes hand, foot and mouth disease (HFMD) in infants and young children. Severe EV71 infections can result in central nervous system complications, and even death. Previous studies suggest that EV71-induced severe complications are associated with programmed cell death and production of pro-inflammatory factors. To determine the molecular morphology and characteristics of EV71-induced cell death, EV71-infected cells were subjected to morphological analysis, JC-1 staining for potential of mitochondrial membrane, fluorescence-activated cell sorting, Annexin V-FITC/PI double staining, and lactate dehydrogenase (LDH) release assay. Western blotting was used to identify the activation of programmed cell death factors, including poly (ADP-ribose) polymerase (PARP), caspase-9, caspase-3, Gasdermin D, and MLKL. The results showed that EV71-infected cells mainly induced apoptotic cell death with a small portion of cellular necrosis. The cleavage of PARP, caspase-9, caspase-3 was observed. Although cell death was mostly inhibited by a caspase inhibitor, cell death still could be observed during the early stage of virus infection. The results suggest that EV71 infection mainly induces apoptotic cell death in combination with a caspase-independent cell programmed death.
白金金,龙健儿. 肠道病毒71型感染诱导细胞程序性死亡形态学及分子生物学特征的初步研究[J]. 微生物与感染, 2018, 13(4): 198-206.
BAI Jinjin, LONG Jianer. Primary study on molecular characteristics and mechanisms of enterovirus type 71-induced programmed cell death. JOURNAL OF MICROBES AND INFECTIONS, 2018, 13(4): 198-206.