新型冠状病毒上海株(nCoV-SH01)的分离和鉴定

doi:10.3969/j.issn.1673-6184.2020.01.004

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微生物与感染 ›› 2020, Vol. 15 ›› Issue (1) : 16-21. DOI: 10.3969/j.issn.1673-6184.2020.01.004

论著

新型冠状病毒上海株(nCoV-SH01)的分离和鉴定

张荣¹，易志刚¹，王玉燕¹，滕峥²，徐巍¹，宋武慧¹，蔡霞¹，孙志平¹，谷陈建¹，周艳秋²，陈洪友²，叶荣¹，韩文东¹，朱云凯¹，冯飞¹，房方皓²，李崇山²，张曦²，瞿涤¹，付晨²，谢幼华¹，袁正宏¹

作者信息 +

Isolation of a 2019 novel coronavirus strain from a coronavirus disease 2019 patient in Shanghai

ZHANG Rong¹, YI Zhigang¹, WANG Yuyan¹, TENG Zheng², XU Wei¹, SONG Wuhui¹, CAI Xia¹, SUN Zhiping¹, GU Chenjian¹, ZHOU Yanqiu², CHEN Hongyou², YE Rong¹, HAN Wendong¹, ZHU Yunkai¹, FENG Fei¹, FANG Fanghao², LI Chongshan², ZHANG Xi², QU Di¹, FU Chen², XIE Youhua¹, YUAN Zhenghong¹

Author information +

文章历史 +

摘要

新型冠状病毒肺炎(coronavirus disease 2019，COVID-19)疫情对人类生命健康构成极大威胁。病毒的分离是构建新型冠状病毒(2019 novel coronavirus, 2019-nCoV)细胞感染模型和动物感染模型的基础。本研究利用冠状病毒易感的Vero E6细胞，从1例上海感染者的咽拭子中分离到一株2019-nCoV，命名为nCoV-SH01。对该毒株全基因组采用一代Sanger和二代Illumina法测序，发现该毒株与GenBank MN908947的同源性＞99.99%。免疫荧光检测显示，该毒株与COVID-19康复者的血清呈阳性反应。当nCoV-SH01感染Vero E6细胞后，导致典型的合胞体病变，细胞病变效应明显且进展迅速，提示nCoV-SH01可用于进一步建立2019-nCoV的细胞感染和动物感染模型，为开展致病性研究以及抗病毒药物和疫苗研发奠定了基础。

Abstract

The spread of 2019 novel coronavirus (2019-nCoV) poses a serious threat to human health. We report here the isolation of a 2019-nCoV strain named nCoV-SH01 from the throat swab from a COVID-19 patient in Shanghai. The isolate grows well on Vero E6 cells with typical syncytial cytopathic effects. The nCoV-SH01 genome has been sequenced using sanger sequencing and Illumina sequencing and analyzed, which shares more than 99.99% identity to a reference sequence (GenBank MN988668 2019-nCoV Wuhan-Hu-1 isolate) with a difference of only three nucleotides. Immunofluorescence results showed that nCoV-SH01-infected cells reacted with the serum of a convalescent COVID-19 patient, suggesting virus replication in cultured cells. The isolation of nCoV-SH01 provides the basis on which cell and animal infection models would be established to facilitate the study of 2019-nCoV pathogenicity and the development of antiviral drugs and vaccines.

导出引用

张荣1，易志刚1，王玉燕1，滕峥2，徐巍1，宋武慧1，蔡霞1，孙志平1，谷陈建1，周艳秋2，陈洪友2，叶荣1，韩文东1，朱云凯1，冯飞1，房方皓2，李崇山2，张. 新型冠状病毒上海株(nCoV-SH01)的分离和鉴定[J]. 微生物与感染. 2020, 15(1): 16-21 https://doi.org/10.3969/j.issn.1673-6184.2020.01.004

ZHANG Rong1, YI Zhigang1, WANG Yuyan1, TENG Zheng2, XU Wei1, SONG Wuhui1, CAI Xia1, SUN Zhiping1, GU Chenjian1, ZHOU Yanqiu2, CHEN Hongyou2, YE Rong1, HAN Wendong1, ZHU Yunkai1, FENG Fei1, FANG Fangha. Isolation of a 2019 novel coronavirus strain from a coronavirus disease 2019 patient in Shanghai[J]. Journal of Microbes and Infections. 2020, 15(1): 16-21 https://doi.org/10.3969/j.issn.1673-6184.2020.01.004

中图分类号： R373