Isolation of a 2019 novel coronavirus strain from a coronavirus disease 2019 patient in Shanghai
ZHANG Rong1, YI Zhigang1, WANG Yuyan1, TENG Zheng2, XU Wei1, SONG Wuhui1, CAI Xia1, SUN Zhiping1, GU Chenjian1, ZHOU Yanqiu2, CHEN Hongyou2, YE Rong1, HAN Wendong1, ZHU Yunkai1, FENG Fei1, FANG Fanghao2, LI Chongshan2, ZHANG Xi2, QU Di1, FU Chen2, XIE Youhua1, YUAN Zhenghong1
1. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), and Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Shanghai Center for Disease Control and Prevention, Shanghai 200336, China
Abstract：The spread of 2019 novel coronavirus (2019-nCoV) poses a serious threat to human health. We report here the isolation of a 2019-nCoV strain named nCoV-SH01 from the throat swab from a COVID-19 patient in Shanghai. The isolate grows well on Vero E6 cells with typical syncytial cytopathic effects. The nCoV-SH01 genome has been sequenced using sanger sequencing and Illumina sequencing and analyzed, which shares more than 99.99% identity to a reference sequence (GenBank MN988668 2019-nCoV Wuhan-Hu-1 isolate) with a difference of only three nucleotides. Immunofluorescence results showed that nCoV-SH01-infected cells reacted with the serum of a convalescent COVID-19 patient, suggesting virus replication in cultured cells. The isolation of nCoV-SH01 provides the basis on which cell and animal infection models would be established to facilitate the study of 2019-nCoV pathogenicity and the development of antiviral drugs and vaccines.
张荣1，易志刚1，王玉燕1，滕峥2，徐巍1，宋武慧1，蔡霞1，孙志平1，谷陈建1，周艳秋2，陈洪友2，叶荣1，韩文东1，朱云凯1，冯飞1，房方皓2，李崇山2，张. 新型冠状病毒上海株(nCoV-SH01)的分离和鉴定[J]. 微生物与感染, 2020, 15(1): 16-21.
ZHANG Rong1, YI Zhigang1, WANG Yuyan1, TENG Zheng2, XU Wei1, SONG Wuhui1, CAI Xia1, SUN Zhiping1, GU Chenjian1, ZHOU Yanqiu2, CHEN Hongyou2, YE Rong1, HAN Wendong1, ZHU Yunkai1, FENG Fei1, FANG Fangha. Isolation of a 2019 novel coronavirus strain from a coronavirus disease 2019 patient in Shanghai. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(1): 16-21.
2020, Vol. 15 
