Isolation and drug resistance of Mycobacterium from clinical specimens of acquired immunodeficiency syndrome patients in Guangxi
MENG Zhihao1, WEI Yongzhong1, LIAO Guangfu1, LU Kangyan1, LU Xueping1, ZUO Yong1, LAN Ke1, TANG Yuelu1, SHEN Yinzhong2
1. Department of Infectious Diseases, Longtan Hospital of Guangxi Zhuang Autonomous Region, Liuzhou 545005, Guangxi Zhuang Autonomous Region, China; 2. Department of Infection and Immunity, Shanghai Public Health Clinical Center Affliated to Fudan University, Shanghai 201508, China
Abstract：To investigate the positive rate, species and drug resistance of Mycobacteria in clinical specimens of acquired immunodeficiency syndrome (AIDS) patients, the AIDS patients in a hospital in Guangxi during January 2010 and December 2019 were collected and their clinical specimens were cultured with acid-fast bacilli. The antimicrobial susceptibility testing was conducted with more than eight anti-tuberculosis drugs after isolation and identification. The overall positive rate was 15.68% (2 163/13 795). The positive rate was high in pus, secretions, various tissue specimens, pleural effusion, and ascites. The species identification results showed that Mycobacteria tuberculosis compound group accounted for 77.95% (1 442/1 850) and non-tuberculosis Mycobacterium (NTM) accounted for 22.05% (408/1 850). The latter accounted for 15.71%-26.07% over 10 years, with no statistically significant difference between years (χ2＝10.442, P>0.05). The overall drug resistance rate was 23.30% (336/1 442), with a significant difference over a 10-year period (χ2＝18.901, P＝0.026). The rates of resistance to isoniazid, rifampicin, streptomycin, ofloxacin, ethambutol, p-amino salicylic acid, kanamycin were 12.14% (175/1 442), 10.54% (152/336), 9.29% (134/1 442), 5.62% (81/1 442), 3.05% (44/1 442), 1.80% (26/1 442), 1.32% (19/1 442), respectively. The prevalence of multidrug-resistant tuberculosis (DR-TB) and extensively drug-resistant tuberculosis (XDR-TB) was 5.48% (79/1 442) and 0.28% (4/1 442), respectively. It is confirmed that the positive rate of Mycobacteria isolated from the clinical specimens of AIDS patients is high and NTM accounts for a high proportion. The drug resistance rate of Mycobacterium tuberculosis to commonly used anti-tuberculosis drugs is high. It is suggested that clinical Mycobacterium identification and antimicrobial susceptibility testing should be carried out to provide a reliable basis for clinical diagnosis and treatment.
蒙志好1，韦永忠1，廖光付1，吕康言1，陆雪萍1，左勇1，蓝珂1，唐月璐1，沈银忠2. 广西地区艾滋病患者临床标本分枝杆菌培养及耐药性分析[J]. 微生物与感染, 2021, 16(2): 103-109.
MENG Zhihao1, WEI Yongzhong1, LIAO Guangfu1, LU Kangyan1, LU Xueping1, ZUO Yong1, LAN Ke1, TANG Yuelu1, SHEN Yinzhong2. Isolation and drug resistance of Mycobacterium from clinical specimens of acquired immunodeficiency syndrome patients in Guangxi. JOURNAL OF MICROBES AND INFECTIONS, 2021, 16(2): 103-109.
2021, Vol. 16 
