Abstract：The present paper aims to analyze the species distribution and drug resistance of nontuberculous mycobacteria (NTM) in Pingdingshan region of Henan Province from 2018 to 2020. A total of 326 NTM strains isolated from Pingdingshan Infectious Disease Hospital from January 2018 to December 2020 were collected. NTM strains were identified by DNA microarray chip, and the modified Lowenstein-Jensen medium was used for drug sensitivity test. The results showed that most of the NTM strains were isolated from the patients aged from 61 to 80 years, followed by the age group of 41 to 60 years. Eight NTM species were identified, namely Mycobacterium intracellulare (35.28%), Mycobacterium chelonae/Mycobacterium abscessus (24.85%), Mycobacterium avium (18.40%), Mycobacterium fortuitum (5.21%), Mycobacterium gordonae (1.23%), Mycobacterium kansasii (12.58%), Mycobacterium gilvum (1.53%), and Mycobacterium scrofulaceum (0.92%). These NTM strains had the highest resistance rate to isoniazid, as high as 97.85%. Except for Mycobacterium gordonae, the resistance rate of NTM strains to isoniazid was ＞94%. Mycobacterium intracellulare had a relatively low resistance rate to prothionamide (8.70%), and the resistance rate of Mycobacterium avium to prothionamide was 10.00%. The resistance rates of Mycobacterium chelonae/Mycobacterium abscessus to isoniazid, rifampicin, streptomycin, ethambutol, and amikacin were ＞95%. Mycobacterium fortuitum had a resistance rate of 35.29% to levofloxacin, and the resistance rate of Mycobacterium kansasii to levofloxacin was 7.32%. The resistance rates of Mycobacterium gordonae to isoniazid, ethambutol, streptomycin and p-aminosalicylic acid were ≥50%. Mycobacterium gilvum had a resistance rate of ＜50% to ethambutol, levofloxacin, amikacin, and kanamycin. Mycobacterium scrofulaceum was not resistant to amikacin and prothiazamide. In conclusion, eight NTM species were found in Pingdingshan region from 2018 to 2020, among which Mycobacterium intracellular accounted for a largest proportion. There are great differences in drug resistance among different NTM species, and the identification of NTM species is of great significance for the treatment of patients.
韦旭. 2018—2020年平顶山地区非结核分枝杆菌菌种鉴定及耐药性分析[J]. 微生物与感染, 2022, 17(2): 65-70.
WEI Xu. Identification and drug resistance analysis of nontuberculous mycobacteria in Pingdingshan region from 2018 to 2020. JOURNAL OF MICROBES AND INFECTIONS, 2022, 17(2): 65-70.
2022, Vol. 17 
