Application progress of metagenomics technology based next-generation sequencing platform in the diagnosis of infectious respiratory diseases pathogens
WANG Huilin, LIU Xue
Department of Respiratory and Critical Care Medicine, The Third People’s Hospital of Zhengzhou, Zhengzhou 450000, Henan province, China
Abstract:The incidence of respiratory infections is high, and early identification of infectious pathogens is the key to improving clinical results. Metagenomics next-generation sequencing (mNGS) has the advantages of being whole spectrum, time saving, no specific amplification of pathogens, and has high clinical application value in the identification of rare infections, mixed infections, immunosuppressive patient infection and the diagnosis of pathogens that are difficult to be detected by conventional methods. However, mNGS results of some cases have low specificity, lack recognized interpretation standards, have unclear relationships between sequencing results and treatment, and have difficulties testing drug-resistant genes. In clinical application present, mNGS and traditional microbial detection technology have a complementary effect and the combination of the two can improve the clinical diagnosis efficiency.
王慧林,刘雪. 宏基因组学二代测序技术在感染性呼吸系统疾病病原体诊断中的应用进展[J]. 微生物与感染, 2021, 16(2): 117-122.
WANG Huilin, LIU Xue. Application progress of metagenomics technology based next-generation sequencing platform in the diagnosis of infectious respiratory diseases pathogens. JOURNAL OF MICROBES AND INFECTIONS, 2021, 16(2): 117-122.