The role of hypoxia inducible factor-1α in inflammation mediated by macrophage infected with H1N1 influenza
HE Yanchao1，2, ZHANG Jin1，2, FENG Jinjin1，2, JIE Zhijun1，2
1. Department of Respiratory Medicine, The Fifth People’s Hospital of Shanghai, Fudan University, Shanghai 200240, China; 2. Center of Community-Based Health Research (Preparation), Fudan University, Shanghai 200240, China
Abstract：To investigate the role of HIF-1α in inflammation mediated by macrophage infect with H1N1(PR8). Mouse macrophages(RAW264.7) were infected with PR8 and sacrificed at 0 h, 6 h, 12 h, 24 h, 48 h post infection(HPI), the mRNA expression levels of HIF-1α， IL-6, TNF-α， M protein(MBP) were determined by real-time PCR. HIF-1α and NF-κB, MAPK, Akt, MBP protein expression levels were detected by Western Blot. In inhibition test, HIF-1α was inhibited by 2-MeOE-2(10 nmol/L), after 24 h, IL-6, TNF-α， MBP mRNA levels and NF-κB, MAPK, Akt, MBP NF-κB, MAPK, Akt protein levels were detected. The results demonstrated that the viral load in infected cells peaked on 24 HPI, HIF-1α mRNA level increased transiently 6 HPI, and peaked on 24 DPI，so it is the same with IFN-γ、IL-6、TNF-α mRNA. After inhibiting HIF-1α in PR8-infected RAW264.7 cells with 2-MeOE-2(10 nmol/L), IL-6、TNF-α mRNA level were decreased when compared with control group (P<0.05). NF-κB protein expression was also decreased when compared with control group. It was concluded that HIF-1α may induce inflammation in H1N1 infection through up-regulating NF-κB pathway of macrophage.
何燕超1，2，张静1，2，冯净净1，2，揭志军1，2. 流感病毒感染巨噬细胞对缺氧诱导因子-1α诱导炎症反应通路的机制研究[J]. 微生物与感染, 2021, 16(3): 164-170.
HE Yanchao1，2, ZHANG Jin1，2, FENG Jinjin1，2, JIE Zhijun1，2. The role of hypoxia inducible factor-1α in inflammation mediated by macrophage infected with H1N1 influenza. JOURNAL OF MICROBES AND INFECTIONS, 2021, 16(3): 164-170.
2021, Vol. 16 
