本文旨在构建LncRNA-GAS5(长链非编码RNA-生长抑制特异性转录本5，long non-coding RNA-growth arrest-specific transcript 5)的真核过表达载体及敲减载体，研究鲍曼不动杆菌对突触体相关蛋白29(synaptosomal-associated protein 29，SNAP29)表达的影响及LncRNA-GAS5在其中发挥的调控作用。采用聚合酶链反应(polymerase chain reaction，PCR)扩增LncRNA-GAS5基因，将其克隆至pcDNA3.1(＋)真核表达载体中，形成pcDNA3.1-GAS5真核表达载体；通过RNA干扰技术，设计敲减GAS5的寡核苷酸序列，将其构建至pRNAT-U6.1/Neo载体中，形成pRNAT-U6.1-GAS5敲减载体；采用实时荧光定量PCR法检测GAS5的表达，以确定是否成功过表达和敲减；采用蛋白免疫印迹法检测过表达或敲减GAS5后HeLa细胞中SNAP29表达的变化，并检测鲍曼不动杆菌感染HeLa细胞后不同时间点SNAP29的表达，以及敲减或过表达GAS5后再感染鲍曼不动杆菌的HeLa细胞中SNAP29的表达。结果显示：pcDNA3.1-GAS5和pRNAT-U6.1-GAS5构建成功；pcDNA3.1-GAS5可使LncRNA-GAS5在HeLa细胞中大量表达，而pRNAT-U6.1-GAS5可使HeLa细胞中GAS5表达明显减少；过表达LncRNA-GAS5可使HeLa细胞中SNAP29表达减少，而敲减LncRNA-GAS5可使SNAP29表达增加；随着鲍曼不动杆菌感染时间延长，HeLa细胞中SNAP29的表达减少，敲减LncRNA-GAS5可使SNAP29表达回复，而过表达LncRNA-GAS5可增强SNAP29的降解。结果提示，本研究成功构建了LncRNA-GAS5的过表达和敲减载体，并发现其对SNAP29表达具有负向调控作用，表明鲍曼不动杆菌对SNAP29的降解依赖LncRNA-GAS5，为进一步探讨鲍曼不动杆菌引起细胞自噬降解障碍的深层机制奠定了理论基础。

The present paper aims to construct the eukaryotic overexpression vector and knockdown vector of LncRNA-GAS5 (long non-coding RNA-growth arrest-specific transcript 5), and to study its role in the expression of SNAP29 in HeLa cells infected by Acinetobacter baumannii (A. baumannii). LncRNA-GAS5 gene was amplified by polymerase chain reaction (PCR) and cloned into pcDNA3.1 (＋) eukaryotic expression vector to form pcDNA3.1-GAS5 eukaryotic expression vector. Through RNA interference technology, the oligonucleotide sequences for knockdown of GAS5 were designed and constructed into pRNAT-U6.1/Neo vector to form pRNAT-U6.1-GAS5 knockdown vector. The expression of GAS5 was detected by real-time PCR to determine whether the overexpression and knockdown were successful or not. Western blotting was used to detect the changes of SNAP29 expression in HeLa cells after overexpression or knockdown of GAS5. In addition, the expression of SNAP29 in A. baumannii-infected HeLa cells at different times and the expression of SNAP29 in A. baumannii-reinfected HeLa cells after knockdown or overexpression of GAS5 were detected by Western blotting. The results showed that pcDNA3.1-GAS5 and pRNAT-U6.1-GAS5 were successfully constructed. pcDNA3.1-GAS5 could induce high expression of LncRNA-GAS5 in HeLa cells. pRNAT-U6.1-GAS5 significantly reduced the expression of GAS5 in HeLa cells. The overexpression of LncRNA-GAS5 decreased the expression of SNAP29 in HeLa cells, while the knockdown of LncRNA-GAS5 increased the expression of SNAP29 in HeLa cells. The expression of SNAP29 decreased progressively with A. baumannii infection time. The knockdown of LncRNA-GAS5 could restore the expression of SNAP29 degraded by A. baumannii, while the overexpression of LncRNA-GAS5 could aggravate the degradation of SNAP29 by A. baumannii. The results suggest that LncRNA-GAS5 has a negatively regulatory effect on SNAP29 expression, and the degradation of SNAP29 by A. baumannii depends on LncRNA-GAS5. It lays a theoretical foundation for further discovering the deep mechanism of autophagy degradation disorder caused by A. baumannii.