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微生物与感染  2023, Vol. 18 Issue (2): 91-96    DOI: 10.3969/j.issn.1673-6184.2023.02.004
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柯萨奇病毒B组3型上海分离株的基因特征分析
李云逸1,杨玉颖1,崔心怡1,费洁2,仲文江3,李淑佩1,周艳秋1,陈敏1
1. 上海市疾病预防控制中心,上海 200336;  2. 上海市嘉定区疾病预防控制中心,上海 201800;  3. 上海市青浦区疾病预防控制中心,上海 201799
Genetic characteristics of coxsackievirus B3 strains isolated in Shanghai
LI Yunyi1, YANG Yuying1, CUI Xinyi1, FEI Jie2, ZHONG Wenjiang3, LI Shupei1, ZHOU Yanqiu1, CHEN Min1
1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China;  2. Jiading District Center for Disease Control and Prevention, Shanghai 201800, China;  3. Qingpu District Center for Disease Control and Prevention, Shanghai 201799, China
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摘要: 为探讨上海市不同来源的柯萨奇病毒B组3型(coxsackievirus B3,CVB3)菌株的循环动态变化及基因特征,本研究对分离自上海市环境污水、健康儿童和急性弛缓性麻痹(acute flaccid paralysis,AFP)病例中的CVB3菌株进行VP1区基因序列测定,并对照全球 CVB3代表株进行序列相似性分析和系统发生学分析。结果显示,1989—2021年上海市不同来源的CVB3分离株共有120株,属于D、E基因亚型。上海CVB3分离株的核苷酸和氨基酸相似性差异较大,分别为78.7%~100%和93.3%~100.0%。2016年首次从上海环境污水中分离到E基因亚型CVB3,2021年再次分离到该基因亚型。2021年上海环境污水中的E基因亚型CVB3分离株与2020年的广东手足口病(hand,foot,and mouth disease,HFMD)病例CVB3分离株VP1 区的核苷酸相似性较高,但上海暂未有该基因亚型感染病例报道。因此,仍须长期对环境污水和不同肠道病毒感染病例进行监测,以提高肠道病毒监测的敏感性,为肠道病毒相关疾病的诊疗提供数据支撑。
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李云逸1
杨玉颖1
崔心怡1
费洁2
仲文江3
李淑佩1
周艳秋1
陈敏1
关键词 柯萨奇病毒B组3型环境污水监测基因特征    
Abstract:To explore the dynamic changes and genetic characteristics of coxsackievirus B3 (CVB3) isolated from different sources in Shanghai, the full VP1 sequences of CVB3 isolates from environmental sewage surveillance, healthy children and acute flaccid paralysis (AFP) surveillance were amplified and sequenced in this study. Similarity and phylogenetic analysis of CVB3 isolates in Shanghai and global representative CVB3 strains were performed. The results showed that there were 120 CVB3 strains isolated from different sources in Shanghai from 1989 to 2021, belonging to D and E genotypes. The similarities of nucleotide and amino acid of Shanghai CVB3 isolates were from 78.7% to 100% and from 93.3% to 100.0%, respectively. In 2016, the genotype E CVB3 was firstly isolated from sewage in Shanghai, and it was isolated again in 2021. The nucleotides of genotype E CVB3 isolates from sewage in 2021 were similar to those of CVB3 isolates from hand, foot, and mouth disease (HFMD) cases in Guangdong in 2020. But, Shanghai has no reported infection cases of genotype E CVB3. It is still necessary to have a long-term surveillance for sewage and enterovirus cases to improve the sensitivity of enterovirus surveillance, and provide data support for the diagnosis and treatment of enterovirus-related diseases.
Key wordsCoxsackievirus B3    Environmental sewage surveillance    Genetic characterization
ZTFEL:  R373  
基金资助:上海市卫生健康委员会卫生行业临床研究专项青年项目(20204Y0210)
通讯作者: 周艳秋,陈敏   
引用本文:   
李云逸1,杨玉颖1,崔心怡1,费洁2,仲文江3,李淑佩1,周艳秋1,陈敏1. 柯萨奇病毒B组3型上海分离株的基因特征分析[J]. 微生物与感染, 2023, 18(2): 91-96.
LI Yunyi1, YANG Yuying1, CUI Xinyi1, FEI Jie2, ZHONG Wenjiang3, LI Shupei1, ZHOU Yanqiu1, CHEN Min1. Genetic characteristics of coxsackievirus B3 strains isolated in Shanghai. JOURNAL OF MICROBES AND INFECTIONS, 2023, 18(2): 91-96.
链接本文:  
http://jmi.fudan.edu.cn/CN/10.3969/j.issn.1673-6184.2023.02.004     或     http://jmi.fudan.edu.cn/CN/Y2023/V18/I2/91

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