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Abstract The aim of this study was to investigate the effect of outer membrane vesicles of Salmonella enterica serovar Typhi(S. Typhi-OMVs) on the proliferation of colorectal cancer cells under different culture conditions. In this study, ultracentrifugation was used to extract the outer membrane vesicles secreted by S. Typhi under the culture conditions of LB, adding acid, hyperosmotic, oxygen stress-derived LB and LPM, respectively. The morphology, particle size and protein distribution under the electron microscope were used to compare S. Typhi-OMVs cultured in different conditions.The colorectal cancer HT-29, SW480 and CT-26 cells were cultured in vitro. CCK-8 detected the effect of S. Typhi-OMVs (acid, hypertonic, oxygen stress and LPM culture conditions) at different time points on the proliferation of colorectal cancer cells, and the clone formation experiment was further verified. The cell cycle was detected by flow cytometry. To evaluate the drug toxicity of S. Typhi-OMVs in mice through body weight and HE staining sections of liver and kidney tissues.The results showed that there was no significant difference in morphology and size of S. Typhi-OMVs under different culture conditions. Compared with other culture conditions, S. Typhi could secrete more outer membrane vesicles under hyperosmotic stress. Under LB and acid stress, hyperosmotic and oxygen stress and LPM culture conditions, the protein distribution of S. Typhi-OMVs was 37~72KDa, 25~72KDa and 8 ~ 55 kDa respectively. S. Typhi-OMVs(LPM culture condition) can significantly inhibit the proliferation of SW480 and CT-26 cells, and arrest the cell cycle of SW480 cells in G2/M phase, and has no obvious hepatorenal toxicity to mice. It is concluded that S. Typhi-OMVs(LPM culture condition) had direct inhibitory effect on the proliferation of colorectal cancer cells (SW480, CT-26), and it was expected to be a therapeutic drug for colorectal cancer.
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Received: 16 March 2023
Published: 25 August 2023
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Corresponding Authors:
Xinxiang Huang
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