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Analysis of drug resistance and homology of Carbapenem-resistant Klebsiella pneumonia isolated from bloodstream infection |
YANG Siyu1, XU Shuibao1, CHEN Zhong2, ZHANG Wenhong1, JIN Jialin1, CHEN Shu1 |
1. Department of Infectious Diseases, HuaShan Hospital, Shanghai 20040, China; 2. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200030, China |
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Abstract Drug resistance and molecular typing of carbapenem-resistant Klebsiella pneumonia(CRKP)isolated from a bloodstream infection was conducted to provide scientific basis for clinical practice, prevention, and control measures. Non-repetitive Klebsiella pneumoniae strains collected in a Shanghai tertiary hospital from April 2015 to March 2018 were subjected to the study. A total of 51 strains of CRKP were isolated. Antimicrobial susceptibility testing was carried out, the carbapenemase genes were detected by PCR, and the homology was analyzed by Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The analysis result showed that the CRKP strains were multi-resistant to antibiotics and blaKPC-2 gene were detected in all strains. Producing blaKPC-2 is a major resistant mechanism to carbapenems for Klebsiella pneumoniae. The results of PFGE typing showed there were dominant clones in this hospital, with ST11 being the main type (84.31%). The results indicated that surveillance of bacterial resistance is an effective control for hospital infection management.
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Received: 26 August 2020
Published: 25 December 2020
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Corresponding Authors:
CHEN Shu
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