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2007 Vol.2 No.4
Published 2007-12-25

述评
论著
Article
讲座
综述
) [HTML 1KB] [PDF 228KB] ( 3796 )
 
论著
197 ZHANG Xing-yi;LI Qun;DING Xing;ZHOU Xin

Antimicrobial resistance of lower respiratory tract infection with Stenotrophomonas maltophilia

Objective To explore antimicrobial resistance of lower respiratory tract infection with Stenotrophomonas maltophilia. Methods The ability of Stenotmphomonas maltophilia, isolated from clinical samples in patients with lower respiratory tract infections, to resist 14 antibiotics was measured by VITEK60 GNS from Jan.2001 - Jan.2007.Results During Jan.2001 - Jan.2007, there were133 Stenotrophomonas maltophilia strains isolated from lower respiratory tract specimens. The sensitivity of Stenotrophomonas maltophilia to the antibiotic Penicillin was the poorest. The sensitivity of Stenotrophomonas maltophilia to Cefepime was the highest as was also the case with approximately 88.0% of the Cephalosporins. However, trimethopsulfamethoxazole seemed to produce the greatest sensitivity (approximately 92.5% ) . Conclusion Stenotrophomonas maltophilia, isolated from the lower respiratory tract, is known to be highly resistance to multiple antibiotics. Trimethoprim-sulfamethoxazolemay may be the best antibiotic choice for Stenotrophomonas rrraltophilia infections. These results also indicate that Clinicans should choose antibiotics according to results attained through an antibiotic susceptibility test.

2007 Vol. 2 (4): 197-198 [Abstract] ( 2689 ) [HTML 1KB] [PDF 181KB] ( 1858 )
199 PAN Wei-hua;LIAO Wan-qing
Analysis on antibiotic resistance of 126 strains of Neisseria gonorrhoeae and an examination of integrated traditional and Western therapies on infection
Objective To monitor antibiotic resistance of Neisseria gonorrhoeae and to test the validity of an integrated traditional and Western medicine therapy regimen. Methods One hundred twenty-six strains of clinically collected Neissena gonorrhoeae were analyzed. A rapid quantitative method of minimum inhibitory concentration (MIC) was used to test 15 anti-microbial agents against 82 strains of Neissena gonorrhoeae, controlled by the Kirby-Bauer, or paper diffusion, method. Patients were treated with ceftriaxone, a third generation cephalosporin antibiotic and traditional medicine. Results There was no difference between the rapid quantitative method and paper diffusion method in detecting antibiotic sensitivity.The antibiotic resistance rate to ciprolloxacin was 75.40% and all strains ( 100%) were sensitive to ceftriaxone. Following the integrated treatment regimen, all patients were cured of their infection. Conclusion The sensitivity of Neisseria gonorrhoeae to ceftriaxone has been steady over the past 6 years, but the antibiotic resistance rate to ciprofloxacin has incresaed. Integrated traditional and Western medicines afforded patients complete recovery from gonorrhea infection.
2007 Vol. 2 (4): 199-201 [Abstract] ( 2409 ) [HTML 1KB] [PDF 256KB] ( 2113 )
202 LIN Wei;YANG Hong-liang;YANG Yang;HU Bao-yu;DONG Ke;TAN Li-zhi;GUO Xiao-kui
Antigenicity and conservation of a novel protein belonging to OmpA family in Leptospira interrogans
Objective To clone and express a novel OmpA gene (LAO301) from Leptospira interrogans , serogroup ictemhaemorrhageae serovar from the Lai strain and to analyze the antigenicity and conservation of this protein from fifteen different strains belonging to 15 prevalent semgmups of L. interrogans from China. Methods The characteristics of LA0301 were predicted by bioinformatics software.The prokaryotic expression system of LA0301 was constructed by routine molecular biological techniques and expression of the recombinant protein was identified by SDS-PAGE and Western blot after induction with IP1'G. Western blot was used to identify the antigenicity and the conservation of the expressed LA030I in fifteen strains from 15 prevalent serogroups in China.The LA0301 antibody from rabbit sera infected with whole leptospires from the Lai strain was identified by Western blot and F.IISA. Results LA0301 is a novel gene belonging to the OmpA family of L. interrogans . The prokaryotic expression system of LAO301 was constructed successfully. The anti-recombinant protein serum titer in BALB/c mouse was 1: 32, 000. The LA0301 protein was detected in all tested strains of L. interrogans . Rabbit serum infected with L. interrogans from the Lai strain was positive for recombinant protein antibody as detected by Western blot and Fl ISA. Conclusion LAO301, a novel gene belonging to the OmpA family, not only displays good antigenicity and conservation but also can elicit antibody responses in rabbits that are infected with L. interrogans from the Lai strain. Our studies on LA0301 provide a basis for developing novel vaccine candidate of leptospira.
2007 Vol. 2 (4): 202-205 [Abstract] ( 3007 ) [HTML 1KB] [PDF 768KB] ( 2039 )
206 MEI Bing;DU Kun; HUO Zhi;WANG Fu-yan;MA Shu-hui;YU Ping
Correlation between MICA* A5.1 allele and infection with Chlamydia trachomatis
Objective To investigate whether there is correlation between an individual' s genetic background and Chlamydia trachomatis (Ct) infection through analyzing the MICA* A5.1 allele and Ct infection in sterile women. Methods MICA* A5.1 alleles were analyzed through the PCR-SSP method, and FQ-PCR was used to detect Ct-DNA. Results Out of the 122 sterile women, 33 were deemed Ct positive. Out of 140 people in the control group, 16 were Ct positive. Therefore, the Ct infection rate was 27.1% and 11.4% in the sterile group and control group, respectively. Among the 122 sterile women, 35 patients were MICA*A5.1 allele positive. In control p, 43 out of 140 people were positive for the MICA* A5.1 allele. Among the sterile women,5 patients were infected with Ct and were positive for the MICA* A5.1 allele simultaneously, whereas only one case in from the control group displayed both factors at the same time.The discrepancy of Ct infection rates between MICA* A5.1 positive cases and MICA* A5.1 negative cases was statistically significant (P = 0.046 in sterile women group; P = 0.022 in control group; P = 0.01 in sterile women patient and control group pooled together) . Conclusion Ct infection rate is higher in sterile women when compared to a control population, and individuals with a positive MICA* A5.1 allele may have a lower susceptibility to Ct infection than ones with a negative allele.
2007 Vol. 2 (4): 206-208 [Abstract] ( 2761 ) [HTML 1KB] [PDF 221KB] ( 1791 )
209 ZOU Xian-biao;ZHANG Guo-wei

An antibody-sandwich enzyme linked immunosorbent assay to detect Ureaplasma urealyticum

Objective To establish an antibody-sandwich enzyme linked immunosorbent assay (ELISA) for the detection of Ureaplasm urealyticum (UU) . Methods We used an antibody sandwich ELISA to detect UU and compared the results with cell culture method. Results The rate of sensitivity of the ELISA was 92.6% and the rate of specificity was 97.4% . The lowest detection limitation was 5~10 ng/ml of UU antigen. Conclusion The antibody-sandwich ELISA is a sensitive, convenient and fast method for detection of UU for the screening of large numbers of specimens.
2007 Vol. 2 (4): 209-210 [Abstract] ( 2226 ) [HTML 1KB] [PDF 191KB] ( 1780 )
211 SUN Chang-jian;CHEN Mei-ling;WANG Xi-le;YANG Xiao;WEN Bo-hai
Efficacy of inactivated Coxiella burnetii against dengue virus infection in mice
Objective To asses the efficacy of the Q fever vaccine against dengue virus infection. Methods The inactivated Coxiella burnetii (whole cell vaccine, WCV) was used to immunize BALB/c mice. For primary immunization, 50ug WCV per mouse was subcutaneously injected and 20ug WCV per mouse was intraperitoneally injected at Weeks 5 and 7 after primary immunization, respectively. One week after the last immunization, each mouse was challenged with 105 TCID50 of dengue virus via administration through the tail vein. The mice were sacrificed and blood and brain samples were collected 48 or 72 h after viral challenge. Samples were then examined by real-time quantitative PCR specific to dengue virus. Results In real-time quantitative PCR assay of blood samples, the amount of virus 72h post-infection was significantly lower than that observed at 48h and no significant difference was found between immunized mice and non-immunized mice. The amount of virus in brain samples from both non-immunized and immunized mice at 48h post infection was very low, however the amount of virus in brain samples from non-immunized mice rose sharply and was significantly higher than that of immunized mice at 72h postinfection.Conclusion Non-specific immunoresponses against dengue vuvs infection may be induced by inactivated C. burnetii in mice.
2007 Vol. 2 (4): 211-214 [Abstract] ( 2868 ) [HTML 1KB] [PDF 841KB] ( 2221 )
219 LV Jian; SUN Zhi; ZHANG Jian-wu; LIU Wei-quan; YUAN Shi-shan
ruction of a full-length infectious clone of PRRSV from

Objective To determine if the JX143 strain of the porcine reproductive and respiratory syndrome virus (PRRSV) is the etiological agent of the "Porcine High Fever Syndrome" outbreak in China. We also sought to test the genetic stability of the eDNA clone and to On insight into the structure and function of the PRRSV genome and the mechanism of it's pathology, which will ultimately provide a is for vaccine development using genetic technologies. Methods Specific primers were designed according to the PRRSV genome. The five cDNA fragments covering the PRRSV genome were amplified and cloned into pBlueScript ⅡSK ( + ) vector. A T7 promoter was introduced immediately upstream of the 5'-end.A molecular marker, Mlu I restriction enzyme site, was introduced in the ORFS coding region. This process resulted in the successful construction of two infectious clones. Results Full-length genomic clones of PRRSV were rescued successfully after transfection into MA104 cells. We then compared the growth properties of the rescued virus and the parental virus using a multi-step growth curve. Animal experiments were executed by inoculating the rescued vines into pigs. All results indicated that there was no difference between the parental and rescued viruses in either their virological and biological properties. Conclusion From this study, we concluded that the etiological agent of the "Porcine High Fever Syndrome" outbreak in China is, in fact, a PRRSV, such as the JX143 strain. This is the first report showing a high virulence virus infectious clone of PRRSV in China.

2007 Vol. 2 (4): 219-227 [Abstract] ( 2971 ) [HTML 1KB] [PDF 605KB] ( 2333 )
 
Article
215 SHAO Guo-qing;LIU Mao-jun;SUN Pei-yuan;WANG Ji-chun ;DU Gai-mei ;ZHOU Yong-qi; LIU Dong-xia
The establishment of an experimental swine model of swine mycoplasma pneumonia

Objective The present study was designed to establish a model of mycoplasma pneumonia of swine ( MPS) by artificial challenge. Methods Mycoplasma hyopneumoniae Is strain was isolated and identified by biochemical and serological assays. MPS negative pigs were inoculated with 2ml of Mycoplasma hyopneumoruae is strain culture by direct lung inoculation. The pigs were observed daily for 15 to 20 days post-inoculation for clinical symptoms and pathological changes. Infected tissues with live Mycoplasma hyopneumoniae virulent strain were lyophilized and used as the master seed for animal challenge. Artificial challenge was performed by delivering 5ml of lyophilized pathological tissues, intratracheally,into 15 Meishan pigs. The lyophilized pathological tissues used for challenge were diluted by 10-2,10-3, 10-4 fold in KM2 culture medium, and pigs in the control group were inoculated with 5 ml KM2 culture medium. Clinical symptoms, pathological changes and lung X-ray results were observed twenty-five days post challenge. Results Typical clinical symptoms and pathological changes associated with MPS were observed in all pigs intratracheally inoculated with lyophilized pathological tissues that were diluted in KM2 culture medium. Therefore, 5 ml of the 10-4 dilution of lyophilized pathological tissues with Mycoplasma hyopneumoniae is strain was the minimum dose required to induce MPS in an artificial challenge model. ConeJusion The artificial challenge model of MPS coWd be established by intratracheally inoculation of 5m1 of a 1o-2 dilution of lyophilized pathological tissues infected with the Myooplasma hyopneumoniae is strain.

2007 Vol. 2 (4): 215-218 [Abstract] ( 2548 ) [HTML 1KB] [PDF 239KB] ( 3112 )
 
讲座
228 HONG Min; LI Qi-han
Virology in the post-genome era
2007 Vol. 2 (4): 228-231 [Abstract] ( 1951 ) [HTML 1KB] [PDF 440KB] ( 1730 )
 
综述
232 ZHANG Li-juan; YU Dong-zheng
Ehrlichiae and ehrlichiosis
2007 Vol. 2 (4): 232-236 [Abstract] ( 1862 ) [HTML 1KB] [PDF 464KB] ( 2177 )
237 YE Ting-lu; LU Chun
Mechanisms of mycoplasma drug resistance to macrolide antibiotics
2007 Vol. 2 (4): 237-239 [Abstract] ( 1638 ) [HTML 1KB] [PDF 261KB] ( 1598 )
240 LI Shao-ping; NIU Yun-tong
Epidemiology and virulence of Candida
2007 Vol. 2 (4): 240-242 [Abstract] ( 1542 ) [HTML 1KB] [PDF 361KB] ( 2071 )
243 WANG Min; HUA Chun-zhen
Roles of virulent factors in the pathogenesis of Hemophilus influenzae
2007 Vol. 2 (4): 243-246 [Abstract] ( 1492 ) [HTML 1KB] [PDF 369KB] ( 1670 )
247 LIU Rui-zi; GAO Qian
Research progress in the interference of antigen presenting cell functions by Mycobacterium tuberculosis
2007 Vol. 2 (4): 247-250 [Abstract] ( 1798 ) [HTML 1KB] [PDF 360KB] ( 1736 )
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