目的 研究2种近交系小鼠在柯萨奇病毒B3型(CVB3)感染后辅助性T细胞(Th)免疫偏离对心肌炎发病的影响。方法 用CVB3腹腔感染BALB/c和C57BL/62种近交系小鼠,感染后7d通过检测小鼠血清肌酸激酶(CK)活性,观察心脏外观变化以及心脏石蜡切片H.E染色观察心脏病理改变,比较2种小鼠心肌炎的发病情况;通过体外感染心肌细胞观察病毒复制情况以及体内心脏组织病毒载量的分析,比较2种小鼠对病毒感染和复制的差异;通过检测感染小鼠细胞因子白细胞介素-4(IL-4)、IL-12和γ干扰素(IFN-γ)的表达,抗CVB3VP1抗体的亚型以及T-bet和Gata-3的表达,比较2种小鼠Th免疫偏离的情况。结果 CVB3在体外和体内都可以感染BALB/c和C57BL/6小鼠心肌细胞,但仅BALB/c小鼠感染后可发生明显的病毒性心肌炎,C57BL/6小鼠则不能;BALB/c小鼠感染后表现为Th1型免疫反应而C57BL/6小鼠则偏向于Th2型免疫反应。结论 CVB3感染2种品系小鼠表现为不同的心肌炎发生率,与其诱导了不同类型的免疫偏离密切相关。

Objective To investigate the contribution of Th immune bias to Coxsackie virus type B3-induced myocarditis on two different mouse strains.Methods BALB/c and C57BL/6 mice were infected intraperitoneally with Coxsackie virus B3.The incidence of myocarditis was evaluated with serum CK activity,appearance and H.E section of the heart tissue.Virus infection and replication were compared between the two mouse strains both in vitro and in vivo.Th immune bias was determined by cytokine expression pattern,antibody isotype and transcription factor T-bet/Gata-3 expression.Results Coxsackie virus B3 infected myocytes from both BALB/c and C57BL/6 mice both in vitro and in vivo.However,only BALB/c mice developed typical myocarditis after infection.Furthermore,BALB/c mice elicited a Th1 type immune response against CVB3 infection while C57BL/6 mice displayed a Th2 type immune response.Conclusion CVB3 infection induced a different pattern of pathological changes in cardiac muscle tissue in two different strains of mice.This difference was correlated with the different Th immune bias induced in these two mouse strains. if(document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');}else{CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}