目的 研究MICA*A5.1基因与沙眼衣原体(Chlamydia trachomatis,Ct)感染的关系。方法 聚合酶链反应(PCR)- SSP检测样本中的MICA*A5.1基因,荧光定量-聚合酶链反应(FQ-PCR)检测Ct-DNA。结果 在122例不孕患者组中有33例Ct感染阳性,140例对照组中有16例Ct感染阳性,2组Ct感染率分别为27.1%和11.4%。经PCR-SSP方法检测,不孕患者组中MICA*A5.1基因阳性个体有35例,Ct感染阳性且同时MICA*A5.1基因阳性的个体有5例;对照组中MICA*A5.1基因阳性个体有43例,Ct感染阳性且同时MICA*A5.1基因阳性的个体有1例。MICA*A5.1基因阳性个体和MICA*A5.1基因阴性个体之间Ct感染率的差异具有统计学意义(不孕患者组P=0.046;对照组P=0.022;两组合并P=0.01)。结论 不孕患者组Ct感染率要高于对照组;与MICA*A5.1基因阴性个体相比,MICA*A5.1基因阳性个体的Ct感染率较低。

Objective To investigate whether there is correlation between an individual' s genetic background and Chlamydia trachomatis (Ct) infection through analyzing the MICA* A5.1 allele and Ct infection in sterile women. Methods MICA* A5.1 alleles were analyzed through the PCR-SSP method, and FQ-PCR was used to detect Ct-DNA. Results Out of the 122 sterile women, 33 were deemed Ct positive. Out of 140 people in the control group, 16 were Ct positive. Therefore, the Ct infection rate was 27.1% and 11.4% in the sterile group and control group, respectively. Among the 122 sterile women, 35 patients were MICA*A5.1 allele positive. In control p, 43 out of 140 people were positive for the MICA* A5.1 allele. Among the sterile women,5 patients were infected with Ct and were positive for the MICA* A5.1 allele simultaneously, whereas only one case in from the control group displayed both factors at the same time.The discrepancy of Ct infection rates between MICA* A5.1 positive cases and MICA* A5.1 negative cases was statistically significant (P = 0.046 in sterile women group; P = 0.022 in control group; P = 0.01 in sterile women patient and control group pooled together) . Conclusion Ct infection rate is higher in sterile women when compared to a control population, and individuals with a positive MICA* A5.1 allele may have a lower susceptibility to Ct infection than ones with a negative allele.