摘要
目的为了提高解脲脲原体(Ureaplasma urealyticum,UU)检测的快速性。方法用酶联免疫吸附试验(ELISA)夹心法检测UU抗原并与传统的培养法相比较。结果ELISA夹心法敏感度为92.6%,特异度为97.4%,最低能够检测出蛋白含量为5～10ng/ml的UU抗原。结论ELISA夹心法是一种敏感、方便、快捷、适合大规模标本检测解脲脲原体的方法。
Abstract
Objective To establish an antibody-sandwich enzyme linked immunosorbent assay (ELISA) for the detection of Ureaplasm urealyticum (UU) . Methods We used an antibody sandwich ELISA to detect UU and compared the results with cell culture method. Results The rate of sensitivity of the ELISA was 92.6% and the rate of specificity was 97.4% . The lowest detection limitation was 5～10 ng/ml of UU antigen. Conclusion The antibody-sandwich ELISA is a sensitive, convenient and fast method for detection of UU for the screening of large numbers of specimens.
关键词
细胞培养 /
酶联免疫吸附试验夹心法
Key words
Ureaplasma urealyticum /
Cell culture /
Antibody-sandwich ELISA
邹先彪;张国威.
酶联免疫吸附试验夹心法检测解脲脲原体方法的建立[J]. 微生物与感染. 2007, 2(4): 209-210
ZOU Xian-biao;ZHANG Guo-wei.
An antibody-sandwich enzyme linked immunosorbent assay to detect Ureaplasma urealyticum
[J].
Journal of Microbes and Infections. 2007, 2(4): 209-210
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