目的 监测我院肠球菌中粪肠球菌株和屎肠球菌株的耐药性，为临床合理应用抗菌药物提供依据。方法 采用法国生物梅里埃公司的GPI板进行细菌鉴定及药敏试验，应用whonet5软件统计粪肠球菌和屎肠球菌的耐药率。结果 粪肠球菌和屎肠球菌对氯霉素、呋喃妥因、万古霉素有较好体外抗菌活性，耐药率都在50%以下，对万古霉素的耐药率在1%以下。粪肠球菌对青霉素、高水平庆大霉素、环丙沙星、利福平、红霉素等大部分抗菌素的耐药率有逐年下降趋势，而屎肠球菌对环丙沙星、利福平、呋喃妥因等抗菌素的耐药率则有上升趋势，屎肠球菌对大多数抗菌素耐药率都高于粪肠球菌。结论 粪肠球菌和屎肠球菌呈多重耐药，临床用药应结合药敏试验结果合理选择抗菌药物。

Objective To investigate the antimicrobial resistance of enterococcus faecalis and enterococcus faecium in our hospital for the rational use of antibiotic. Methods GPI plate was applied for bacterial identification and susceptibility testing.Whonet5 software was used for data analysis. Results Less than 50% of enterococcus faecalis and enterococcus faecium isolates were resistant to chloramphenicol and nitrofurantoin. Less than 1% of enterococcus faecalis and enterococcus faecium isolates were resistant to vancomycin. The resistance rate of enterococcus faecalis to penicillin G, high concentration gentamicin, ciprofloxacin, rifampicin and erythromycin descend year by year, and that of enterococcus faecium to ciprofloxacin, rifampicin and nitrofurantoin was opposite. In addition, the resistance rate of enterococcus faecium to most antibiotic was higher than enterococcus faecalis. Conclusions The enterococcus faecalis and enterococcus faecium is becoming multi-resistant bacteria. Antimicrobial therapy should be decided according to the results of susceptibility testing.