结核分枝杆菌Rv2629基因产物的亚细胞定位和穿梭质粒转化耻垢分枝杆菌

王庆忠1,2; 徐颖1; 陈嘉臻1; 祝秉东1; 郄亚卿1; 王九龄1; 王洪海1

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微生物与感染 ›› 2008, Vol. 3 ›› Issue (2) : 90-93.

论著

结核分枝杆菌Rv2629基因产物的亚细胞定位和穿梭质粒转化耻垢分枝杆菌

王庆忠1，2; 张鹭1; 徐颖1; 陈嘉臻1; 祝秉东1; 郄亚卿1; 王九龄1; 王洪海1

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Subcellular location of Rv2629 in M. tuberculosis and its transformed version into M. smegmatis using the pMV261 plasmid

WANG Qing-zhong1,2; ZHANG Lu1; XU Ying1; CHEN Jia-zhen1; ZHU Bing-dong1; QIE Ya-qing1; WANG Jiu-ling1; WANG Hong-hai1

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摘要

目的研究结核分枝杆菌利福平耐药相关蛋白Rv2629在细胞内的亚定位及其与药敏的相关性。方法采用差速离心进行细胞组分分离及Western-blot检测初步判定蛋白亚细胞定位；采用pMV261转化耻垢分枝杆菌，BACT MGIT960测定转化菌株的利福平耐受性。结果 Rv2629蛋白主要定位于结核分枝杆菌的细胞壁和细胞膜，重组有Rv2629 突变位点191C质粒的耻垢分枝杆菌对利福平的MIC为160mg/L，相应的携带有野生型基因191A的宿主菌MIC为20mg/L。结论 Rv2629基因191A/C突变同利福平耐药相关。

Abstract

Objective To detect the subcellular location of Rv2629 in Mycobactrium tuberculosis, and the drug susceptibility testing of transformed M. smegmatis. Methods Different component of Mycobacterium tuberculosis were separated by differential velocity centrifugation, and subcellular location of Rv2629 was examined by Western-Blot. pMV261 plasmid was used to transformed the Rv2629 gene to M. smegmatis. RIF resistant was assayed by BACTEC MGIT 960 instrument. Results The subcellular location of Rv2629 was major at cell wall and cell membrane. The MIC of RIF for M. smegmatis transformed with mutated Rv2629 was 160μg/mL. While the MIC for wild type Rv2629 gene was 20μg/mL similar to the parental strain. Conclution These results indicate that the 191A/C mutation of Rv2629 gene is associated with RIF resistance.

导出引用

王庆忠1，2; 张鹭1; 徐颖1; 陈嘉臻1; 祝秉东1; 郄亚卿1; 王九龄1; 王洪海1. 结核分枝杆菌Rv2629基因产物的亚细胞定位和穿梭质粒转化耻垢分枝杆菌[J]. 微生物与感染. 2008, 3(2): 90-93

WANG Qing-zhong1,2; ZHANG Lu1; XU Ying1; CHEN Jia-zhen1; ZHU Bing-dong1; QIE Ya-qing1; WANG Jiu-ling1; WANG Hong-hai1. Subcellular location of Rv2629 in M. tuberculosis and its transformed version into M. smegmatis using the pMV261 plasmid[J]. Journal of Microbes and Infections. 2008, 3(2): 90-93