为了筛查结核分支杆菌等细菌的L型细菌，我们将2008年9月至2009年9月于兰州市肺科医院就诊的340例痰培养标本分别接种于罗氏培养基、Middlebrook MGIT-960液体培养基、含和不含氯化钠的92-3TB液体培养基以及含和不含蔗糖的Middlebrook 7H9半固体培养基中，观察其菌落生长状况。其中一株（痰标本编号为866）在罗氏培养基（LJ）、Middlebrook MGIT-960、不含氯化钠的92-3 TB（92-3 TB-B）液体培养基以及不含蔗糖的Middlebrook 7H9（7H9-B）半固体培养基不生长，而在含蔗糖的7H9（7H9-L）半固体培养基上呈煎蛋样嵌入性生长，在含氯化钠的92-3TB(92-3 TB-L)液体培养基也生长。涂片抗酸染色镜下观察可见大小不等的抗酸阴性球形菌。传代培养两代后可见橘红色菌落，涂片抗酸染色可见少量抗酸染色阳性杆状菌，电镜观察见菌体为杆状。PCR扩增16SrRNA DNA，经序列比对分析发现该菌株为放线菌目戈登氏菌属的支气管戈登菌(Gordonia bronchialis, G. bronchialis）。G. bronchialis存在于环境中，在机体免疫抵抗力下降时可致病。该患者78岁，间断性咳嗽咳痰四年，多次给予抗炎利痰治疗，为条件致病菌感染创造了条件。结论：我们发现一个老年患者肺部感染了L型支气管戈登菌，提示肺部感染迁延不愈的老年患者需要考虑感染L型支气管戈登菌的可能。

In the process of screening for L-form mycobacteria, 340 sputum specimens during the period from September 2008 to September 2009 in Lanzhou Pulmonary Hospital were cultured on LJ medium, Middlebrook MGIT-960， 92-3 TB liquid medium with or without sodium chloride and Middlebrook 7H9 semi-solid medium with or without sucrose, then the growth condition and the morphology of colony were observed. One bacterial strain numbered 866 had embedded growth on 7H9-L semi-solid as fried egg colonies, which was acid fast negative and round under optical microscope. After subculture, the colony showed pinkish and reddish color, the bacteria appeared as rod in optical and scanning electron microscopes (SEM)，and there were a few positive rod bacteria by acid fast staining. 16S rRNA DNA sequence analysis revealed that the strain belonged to Gordonia bronchialis series of Gordona genus in Actinomycosis order. The patient，78-year-old, had coughed and expectorated sputum intermittently for four years with some sigh of deterioration of immunity. In conclusion, the elder patient might be infected with L-form of Gordonia bronchialis, which may suggest that Gordonia bronchialis L-form infection could underlie persistent lung infection in elderly patients.