本文通过对上海市疾病预防控制中心收集并保存的、来源于上海崇明岛地区痰培养阳性肺结核患者的135 株结核分枝杆菌北京基因型菌株进行可变数目串联重复序列( VNTR) 和单核苷酸多态性( SNP) 基因分型, 并构建最小生成树( MST) , 来寻找适合研究北京基因型菌株微进化的VNTR 组合及区分不同亚型的分子标志。结果显示,16 位点VNTR 与SNP 的分型结果最匹配, 适合于研究该地区北京基因型菌株的微进化;SNP 单倍型主要集中在ST10( 42 .2%) 、ST19( 30 .4%) 、ST22( 14 .1 %) 3 个类型;4 个VNTR 位点( Mtub21 、QUB26 、MIRU16 和QUB4156) 可能是区分北京基因型菌株不同亚型的分子标志。

To find the variable number of tandemrepeat ( VNTR) locus combinations that adapt to study the microevolution of Mycobacterium tuberculosis ( M. tuberculosis ) strain of Beijing genotype and the molecular markers to distinguish diff erent sublineages , VNTR and single nucleotide polymorphism( SNP) genotyping were usedto genotype 135 strains fromculture-positive sputumof tuberculosis patients ( collected and preserved by Shanghai Municipal Center f or Disease Control and Prevention) in Chongming Island , Shanghai . Based on the data, the mini mumspanning tree ( MST) was constructed . The results showed that the genotyping inf ormation of 16-locus VNTR best matched the SNP genotyping data, indicating that it was applicable in the microevolution study of M. tuberculosis strain of Beijing genotype in Chongming Island ,
Shanghai . SNP hapl otypes were mainly concentrated in ST10 ( 42. 2%) , ST19 ( 30. 4%) , and ST22 (14.1 %) . Four VNTRloci ( Mtub21 , QUB26 , MI RU16 and QUB4156) may be the molecular markers to distinguish different sublineages of Beijing genotype strains .