本文旨在研究临床分离酵母的种类及药物敏感性。采用CHROMagar Candida 显色培养基和API 20C AUX 对我院2008 年1 ～12 月分离自临床深部真菌感染患者的1386 株酵母进行鉴定, 并采用Rosco 纸片扩散法分析其对两性霉素B、氟康唑、伊曲康唑、制霉菌素、伏立康唑、5- 氟胞פ、克霉唑的药物敏感性, 数据用Whonet5 .4 软件分析。结果显示, 分离出的1 386 株酵母中, 白假丝酵母占58.95%, 热带假丝酵母占15 .95 %, 光滑假丝酵母占15 .15 %, 克柔假丝酵母占2 .74 %, 其他酵母占7 .21 %。葡萄牙假丝酵母、近平滑假丝酵母、白假丝酵母、热带假丝酵母、光滑假丝酵母和克柔假丝酵母对氟康唑的敏感率分别为95.45 %、94 .29 %、82.99%、79.19 %、68 .57 %和28.95%; 白假丝酵母对5- 氟胞פ、两性霉素B、制霉菌素、伏立康唑、氟康唑、伊曲霉唑和克霉唑的敏感率分别为93 .76 %、93. 15 %、84 .58 %、84 .09 %、82 .99 %、80 .05% 和78 .09 %。结果提示, 我院深部真菌感染仍以白假丝酵母为主, 其次是热带假丝酵母、光滑假丝酵母; 白假丝酵母对5- 氟胞פ、两性霉素B 的敏感率最高; 除克柔假丝酵母外的所有酵母对氟康唑的敏感率都很高。

The present paper ai ms to study clinical yeast isolates and analyze their susceptibility to antifungal drugs . API 20C AUXidentification systemand CHROMagar Candida mediumwere used to i dentify a total of 1 386strains of yeast collectedin our hospital fromJanuary to December , 2008 . The Rosco disk diffusion method was used to test the susceptibility of yeast to amphotericin B, fluconazole , itraconazole , nystatin , voriconazole , 5-flucytosine , and clotri mazole . The data were analyzed by Whonet 5 .4 software . Of the 1 386 strains collected, Candida albicans ( C. albicans) was the most frequent ( 58 .95%) , f oll owed by C. t r opicalis ( 15 .95 %) , C. glabr at a ( 15 .15 %) , C. kr usei ( 2.74%) , and others ( 7.21%) . Antifungal susceptibility testing results showed that the sensitive rates of C. l usitaniae , C. par apsilosi s , C. al bicans ,
C. t r opicalis , C. glabr at a , and C. kr usei to fluconazole were 95 .45 %, 94 .29 %, 82.99%, 79.19 %, 68 .57 % and 28 .95 %, respectively . The sensitive rates of C. albicans to 5-flucytosine , amphotericin B, nystatin, voriconazole , fluconazole , itraconazole , and clotri mazole were 93 .76%, 93 .15 %, 84.58 %, 84 .09 %, 82.99 %, 80 .05 % and 78.09 %, respectively . These results suggest that the major pathogen causing deep fungal infection in our hospital is C. al bicans , f ollowed by C. tr opicalis and C. glabr ata . C. al bicans was most susceptible to the antifungal eff ects of 5- flucytosine and amphotericin B. All of the yeast isolates collected, except f or C. kr usei , were highly sensitive to fluconazole .