乙型肝炎病毒( HBV) X 蛋白( HBx) 与HBV 相关肝细胞肝癌( HCC) 的发生和发展密切相关。深入研究HBx 在HCC 形成中的作用将为探索HBV 致癌机制提供重要依据。HBx 是多功能蛋白, 其对细胞凋亡的影响至今仍存在分歧。许多研究表明,HBx 既有促进细胞凋亡又有抑制细胞凋亡的功能, 但原因不清楚。本研究中, 将表达HBx 的质粒短暂转染Huh7 和293T 细胞, 结果发现HBx 对细胞凋亡的影响存在剂量依赖效应。转染低剂量HBx 表达质粒会抑制细胞凋亡发生, 而当转染量达到较高程度( >1 μg) 时, 反而会促进细胞凋亡。本实验初步探索了HBx 剂量依赖调节细胞凋亡的作用机制, 发现HBx 随着表达量的不同会影响核因子κB( NF-κB) 的细胞定位。

Hepatitis B virus Xprotein ( HBx) plays a role in the development of hepatocellular carcinoma ( HCC) . Studies on t he relationshi p bet ween HBx and HCC development can provide an important basis for the mechanism of tumorigenesis of HBV. Amongits various activities , HBx can regulate cell apoptosis and proliferation . However , whether HBx is anti-apoptotic or pro-apoptotic is still debatable . In this study , Huh7 and 293Tcells were transiently transfected with HBx-expressing plasmid at different concentrations . The results showed that HBx could exhibit anti- or pro-apoptotic activity depending on the transfection concentration. At a low transfection concentration, HBx appeared to inhibit apoptosis while it promoted apoptosis at a higher transfection concentration. Furt her more , preli mi nary results suggested that different HBx expression levels could cause different cellular localizations of nuclear fact or κB( NF-κB) .