为了解健康产妇顺产新生儿单纯疱疹病毒（HSV）感染情况，自2010年11月~2011年6月于某地妇幼保健院采集顺产新生儿抗凝脐带血51份。提取新生儿脐带血单核细胞DNA后，利用HSV-1 gD基因和HSV-2 gG基因特异性引物，进行套式聚合酶链反应（PCR）检测，并将阳性扩增产物测序后进行BLAST比对确认，分析HSV感染阳性率。结果显示，51份脐带血HSV-1阳性12份，感染率23.53%； HSV-2阳性13份，感染率25.49%；HSV-1和HSV-2共阳性4份，重复感染率7.84%。结果表明，加强新生儿HSV感染的早期诊断和治疗，对预防新生儿HSV感染所致疾病，防止后遗症具有重要意义。

The present paper aims to investigate the herpes simplex virus (HSV) infection in neonates. From November 2010 to June 2011, fresh neonatal cord blood was collected from 51 neonates in a Maternity and Children Hospital and transported immediately to our laboratory. After DNA extraction from the samples, nested-polymerase chain reactions (PCR) were performed by using HSV-1 gD gene- and HSV-2 gG gene-specific primers to detect congenital HSV-1 and HSV-2 infections respectively. The PCR products were sequenced toconfirm the HSV infections.. The results indicated that the infection rate of HSV-1 and HSV-2 were 23.53% and 25.49% respectively. The HSV-1/HSV-2 co-infection rate was 7.84%. This study suggests that early diagnosis and treatment of neonatal HSV infection is critical for the prevention of diseases caused by HSV infection.