生物安全实验室微环境消毒是控制实验室污染的重要环节。过氧化氢广泛用于病原微生物实验室微环境消毒，但其对不同病原微生物的消毒效果有待研究。本文研究了过氧化氢干雾（粒径<10 µm）不同消毒程序对生物安全柜表面常见微生物的消毒效果。结果显示，在生物安全柜内采用优化的消毒程序（发散循环8次，每次1 min，达60 ppm后，静置消毒2 h），利用该程序，过氧化氢干雾可完全杀灭1×10⁶ CFU枯草芽胞杆菌、嗜热脂肪芽胞杆菌、金黄色葡萄球菌、表皮葡萄球菌、耻垢分枝杆菌，以及1×10⁸ CFU大肠埃希菌。然而，当金黄色葡萄球菌、表皮葡萄球菌、耻垢分枝杆菌微生物浓度达1×10⁷ CFU时，过氧化氢干雾无法完全杀灭微生物。因此，建议在进行过氧化氢干雾消毒时，应先用消毒剂处理，以期彻底杀灭生物安全柜微环境中污染的病原微生物。

Disinfection of microenvironment surface is very important for control contamination of biosafety laboratory. Hydrogen peroxide has been used widely in biosafety laboratories for disinfection. The effect of hydrogen peroxide on inactivation of different bacteria was investigated in the present study. The effect of hydrogen peroxide in the form of dry-mist (diameter <10 µm) on sterilization of common pathogens in the biosafety cabinet (BSC) was evaluated. The optimized procedure (diffusing 1 min for 8 cycles, reaching 60 ppm, and staying 2 h) has been determined, at which the hydrogen peroxide dry-mist was able to completely kill 6 logs of Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Mycobacterium smegmatis (M. smegmatis), Bacillus subtilis (B. subtilis) and Bacillus stearothermophilus (B. stearothermophilus), as well as 8 logs of Escherichia coli (E. coli), but not 7 logs of S. aureus, S. epidermidis and M. smegmatis. Therefore, it is suggested that before disinfection of BSC surface microenvironment by hydrogen peroxide dry-mist it is necessary to wipe the surface of BSC or decontaminate extravasation spilth with disinfectants.