本研究原核表达并纯化了布鲁氏菌脂蛋白Omp19，并通过蛋白免疫印迹法对其免疫原性进行验证。采用Omp19作为包被抗原，建立检测羊种布鲁氏菌的间接酶联免疫吸附试验（iELISA），检测90份羊血清样本，并与标准血清凝集试验（SAT）比较，用SPSS软件进行数据分析。结果显示，iELISA与SAT的阳性符合率为95.12%，阴性符合率为67.35%，Kappa值为0.607 7。对2种方法进行McNemar 卡方检验，结果有显著性差异（P<0.05）。本研究建立的iELISA与SAT的总符合率达80%，可作为羊种布鲁氏菌血清学诊断的备用方法。

Brucella protein Omp19 was Expressed and purified, and its immunogenicity was further validated by Western blotting. An indirect enzyme-linked immunosorbent assay (iELISA) for detection of Brucella melitensis (B. melitensis ) was established using the purified recombinant protein Omp19 as coating antigen. Serum samples (n=90) from sheep were simultaneously tested by iELISA and standard serum agglutination test (SAT), and the data was analyzed by SPSS software. The results showed that compared with SAT the positive coincidence rate of iELISA was 95.12% and the negative coincidence rate was 67.35%, Kappa value was 0.607 7 . McNemar chisquare test showed that there was significant difference between the two methods (P<0.05). The total diagnosis coincidence rate reached 80%. The established iELISA could be used as an alternative method for serological diagnosis of brucellosis.