为在体外评价马纳维诺凝胶的生物学活性和稳定性，用TZM-bl和Jurkat-T细胞系对马纳维诺与0.015%羟乙基纤维素（HEC）复合而成的凝胶制剂进行细胞毒性检测，评价其抑制人类免疫缺陷病毒（HIV）SVPB16和SVPC12亚型假病毒感染的活性，并与溶于磷酸盐缓冲液（PBS）的对照组作比较。将马纳维诺溶于1.5% HEC中，终浓度为6 mmol/L，分别置于4 ℃、室温、30 ℃和40 ℃，保持相对湿度75%，每隔1周检测其抗病毒活性。结果显示，当马纳维诺与HEC复合后，其细胞毒性并没有增加，而抗病毒活性轻微增强。马纳维诺凝胶置于4个温度，持续8周，即使是在高于人体温度的40 ℃，仍保持较好的抗病毒活性。综上所述，作为候选杀微生物剂，马纳维诺凝胶具有较好的生物学活性和稳定性。

To study the efficacy and stability of maraviroc gel in vitro, the cytotoxicity of maraviroc in phosphate buffered saline (PBS) or in 0.015% hydroxyethyl cellulose (HEC) were evaluated in TZM-bl and Jurkat-T cells. Meanwhile, two subtypes of human immunodeficiency virus (HIV) pseudovirus (SVPB16 and SVPC12) were used to evaluate the anti-HIV activity in vitro, the half maximal inhibitory concentration (IC₅₀) or 90% maximal inhibitory concentration (IC₉₀) of maraviroc gel were assessed. Furthermore, maraviroc was dissolved in 1.5% HEC with a final concentration of 6 mmol/L, and then maraviroc gel was stored at 4 ℃, room temperature, 30 ℃ and 40 ℃, respectively, in 75% relative humidity for 1 to 8 weeks. The anti-HIV activity was tested weekly. The data showed that maraviroc gel had nearly same cytotoxicity as maraviroc in PBS. HEC did not weaken the ant-HIV activity of maraviroc. Inversely, HEC might slightly enhance the anti-HIV efficacy of maraviroc. Maraviroc gel was stable for at least 8 weeks even at 40 ℃. It is concluded that maraviroc gel has good efficacy and stability as a microbicide candidate.