为初步研究树突细胞特异性细胞间黏附分子-3结合非整合素因子（DC-SIGN）是否能作为严重发热伴血小板减少综合征病毒（SFTSV）的细胞表面受体，首先通过实时反转录-聚合酶链反应（RT-PCR）检测不同细胞系DC-SIGN mRNA水平及SFTSV感染水平，并用DC-SIGN特异性单克隆抗体封闭SFTSV敏感细胞，研究其对SFTSV感染水平的影响。此外，将DC-SIGN表达载体转染不同细胞系，研究转染前后细胞中SFTSV感染水平的变化。结果显示，在SFTSV感染水平较高的Vero细胞中，DC-SIGN mRNA水平较高；而在SFTSV感染水平低于Vero细胞的CHO-K1细胞中，DC-SIGN mRNA水平较低。DC-SIGN单克隆抗体在一定程度上能抑制病毒进入宿主细胞，并呈现剂量-效应关系，DC-SIGN可提高Vero和CHO-K1细胞中SFTSV的感染水平。本研究显示DC-SIGN为SFTSV的可能受体。

To study cell surface receptors of severe fever with thrombocytopenia syndrome virus (SFTSV), the levels of dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) mRNA and SFTSV infection in different cell lines were identified by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). SFTSV-sensitive cells were inhibited by anti-DC-SIGN monoclonal antibody (mAb) and virus infection levels were detected by real-time RT-PCR. Different cell lines were transfected with DC-SIGN-expressing plasmid and SFTSV infection levels either before or after transfection were detected. The results showed that Vero cells which were sensitive to SFTSV had a relatively higher DC-SIGN level than CHO-K1 cells which were less sensitive to SFTSV. Anti-DC-SIGN mAb was capable to inhibit SFTSV invasion into host cells to a certain extent, showing a dose-response effect. The levels of SFTSV infection in cells transfected with DC-SIGN-expressing plasmid were higher than those in non-transfected cells. It is suggested that DC-SIGN is probable one of the cell surface receptors of SFTSV.