中东呼吸综合征(MERS)是一种由中东呼吸综合征冠状病毒(MERS­-CoV)感染所致的急性呼吸道传染病，其临床诊断需依据实验室病原学检测结果。本文就MERS­CoV的核酸检测、抗原和抗体检测、病毒分离鉴定及基因变异监测等方面的最新研究进展作一综述。

Middle East respiratory syndrome (MERS) is an acute respiratory infectious disease caused by Middle East respiratory syndrome coronavirus (MERS­-CoV). Its clinical diagnosis is based on pathogen detection in the laboratory. In this review, we summarize the latest research progress on the methodology of nucleic acid detection, serological detection, virus isolation and genetic variation monitoring of MERS-­CoV.