为提高DNA疫苗的体内转化效率，增强抗原的体内表达，本研究选用慢病毒来源的表达载体pFUW构建新型重组DNA质粒pFUW-Ag85B-Rv3425。用重组质粒DNA对C57BL/6小鼠进行肌内免疫后，立即利用电脉冲核酸药物导入仪在DNA免疫部位施以电压的免疫策略，第3次免疫2周后检测免疫效果。结果显示，新型重组DNA质粒pFUW-Ag85B-Rv3425在电击介导下能刺激机体产生更强烈的体液免疫反应，细胞免疫倾向于Th1 型免疫反应，显著增强CD8⁺ T细胞和CD27⁺ B细胞增殖。研究表明，电击介导重组DNA质粒pFUW- Ag85B-Rv3425免疫小鼠能增强免疫效果，有望成为新型的结核分枝杆菌疫苗免疫策略。

A novel DNA vaccine pFUW-Ag85B-Rv3425 based on lentiviral vector pFUW was constructed to improve the transfection efficiency in vivo. The DNA injection combined with electroporation in C57BL/6 mice three times with two-week interval was conducted. After the last immunization, the mice were sacrificed. The spleen lymphocytes were isolated and analyzed by flow cytometry. The expression levels of IgG, IgG1, IgG2a, and interleukin 2 (IL-2) in serum were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that DNA injection in combination with electroporation could induce stronger humoral immunity, more Th1-type immune response and significantly enhance CD8⁺ T cell and CD27⁺ B cell proliferation. It is indicated that DNA vaccine in combination with electroporation can be taken as a new therapy strategy against tuberculosis.