在高致病性病原微生物实验活动过程中，选择有效的消毒剂对实验室环境去污染非常重要，这也是避免实验室获得性感染发生的有效途径。本研究针对结核分枝杆菌H37Rv，应用滤膜过滤悬液定量杀菌实验进行消毒剂验证，其中75%乙醇、含有效氯1 000 mg/L的次氯酸钠及Safespore消毒剂可高效杀灭结核分枝杆菌，将37.5%乙醇作用延长至10 min也可有效杀灭结核分枝杆菌，这为选择针对结核分枝杆菌的生物安全三级实验室表面消毒剂提供了依据。

The application of effective disinfectants during experimental activities of high­pathogenic microorganism is crucial for decontamination of laboratory environment to prevent laboratory­acquired infections. In this study, the quantitative germicidal disinfectant validation experiments using membrane filtration suspension were conducted against Mycobacterium tuberculosis (M. tuberculosis) H37Rv. Sodium hypochlorite solution (containing 1 000 mg/L active chlorine), 75% ethanol/water, and Safespore disinfectant killed M. tuberculosis efficiently. When the concentration of ethanol decreased to 37.5%, it was still able to kill M. tuberculosis by prolonging killing time to 10 min. The results suggest that 75% ethanol/water is suitable as conventional disinfectant and sodium hypochlorite solution (containing 1 000 mg/L active chlorine) is suitable under emergency and accident in biosafety level 3 laboratories (BSL­3)．