本研究旨在了解和掌握狂犬病、弓形虫和A型流感在社区流浪猫中的免疫和流行状况，为社区流浪猫人兽共患病公共卫生防控提供依据。采用实时荧光聚合酶链式反应（PCR）检测流浪猫全血样本中弓形虫核酸，以及咽喉和肛门拭子样本中流感病毒核酸。本研究对2022年6月至2023年8月在上海青浦地区40个小区采集的153只流浪猫血清样品开展狂犬病、弓形虫和A型流感病毒抗体检测，并对A型流感抗体阳性样品开展H3、H5、H7和H9分型鉴定。结果显示153份流浪猫血清样品狂犬病、弓形虫和A型流感抗体阳性率分别为14.38%（22/153）、11.76%（18/153）和3.92%（6/153）。春季（3-5月）和夏季（6-8月）弓形虫血清抗体阳性率差异极显著。检出H3亚型抗体阳性样品1份，阳性率0.65%（1/153）。研究表明上海地区流浪猫狂犬病免疫抗体水平不足以阻断狂犬病病毒传播。流浪猫群体存在弓形虫感染，尤其在夏季传播风险更高。流浪猫存在A型流感病毒感染，提示流浪猫可以成为人和动物流感病毒的“混合器”，造成人兽共患病传播风险。因此，有必要对流浪猫群体开展狂犬病、弓形虫、A型流感病毒感染情况持续监测，为科学防控提供依据。

To understand the prevalence of rabies, Toxoplasma gondii and influenza A virus in stray cats in Qingpu, Shanghai. Serum samples from 153 stray cats collected from 40 residential community between June 2022 and August 2010 were tested for antibodies to rabies, Toxoplasma gondii and influenza A viruses by ELISA. The H3, H5, H7 and H9 serotypes of influenza A antibody positive samples were identified by HI test. Real-time PCR was used for the detection of Toxoplasma gondii RNA in whole blood samples and influenza virus RNA in throat and anal swab samples. The results showed that the positive rates of rabies, Toxoplasma gondii and influenza A were 14.38% (22/153) , 11.76% (18/153) and 3.92% (6/153) respectively. The difference of positive rate of Toxoplasma gondii antibody between spring (March-May) and summer (June-August) was very significant. The positive rate of H3 was 0.65% (1/153) . No H5, H7 and H9 antibody was detected. The results showed no Toxoplasma gondii antigen was detected in the whole blood samples ,and no influenza antigen was detected in throat swab samples and anal swab samples. The results suggest that the rabies antibody level of stray cats in Qingpu District was low, which was not enough to block the Rabies transmission. Toxoplasma gondii infection exists in stray cats, especially in summer. The presence of influenza A infection in stray cats suggests that stray cats can act as a "Mixer" for human and animal influenza viruses, posing a risk of zoonosis transmission. Therefore, it is necessary to continuously monitor the infection of rabies, Toxoplasma gondii and influenza A virus in stray cats to provide basis for scientific prevention and control.