本研究旨在了解上海市疾控机构病原微生物实验室的现况，对实验室的生物安全状况进行分析，为进一步加强和规范上海市实验室生物安全管理提出对策建议。采用现场调查的方式对上海市16家区级疾控机构的病原微生物实验室生物安全状况进行调查。发现实验室设施设备配备及运行指标中，生物安全柜、高压灭菌器、洗眼器、紫外灯的配置率达到100%；设备运行维护指标中，生物安全柜和紫外灯维护情况较好，洗眼器和高压灭菌器的维护合格率为87.50%；生物安全管理制度的实施指标中，管理制度的指标完成较好，人员管理和实验过程管理的指标还需提升，废水处置余氯监测值指标符合率低于80%。结果显示上海市疾控机构病原微生物实验室在生物安全制度建立、实验室责任落实等环节完成得较好，在废弃物处置、设施设备管理、应急预案制度等方面还需要进一步完善和提高。

The purpose of the current study is to investigate the current situation of pathogenic microorganism laboratory in Shanghai disease control institutions, analyze the biosafety situation of the laboratory, and put forward countermeasures and suggestions for further strengthening and standardizing the biosafety management of laboratories in Shanghai. Investigate the biosafety status of pathogenic microorganism laboratories in 16 district level disease control institutions in the field testing. It was found that the allocation rate of biosafety cabinet, autoclave, eye washer and UV lamp reached 100%. The biosafety cabinet and UV lamp are well maintained and the qualified rate of eye washer and autoclave is 87.50%. Among them, the indicators of the management system have been completed well, and the indicators of personnel management and experimental process management need to be improved. The compliance rate of residual chlorine monitoring values in wastewater treatment is lower than 80%. The results show that the establishment of biosafety system and the implementation of laboratory responsibilities are well completed, and waste disposal, facilities and equipment management, and emergency plan system need to be further improved and improved.