为探讨影响金黄色葡萄球菌杀白细胞素ED(leukocidin ED，LukED)转录表达的最佳培养条件，采用定量反转录-聚合酶链反应(quantitative reverse transcription-polymerase chain reaction，qRT-PCR)检测金黄色葡萄球菌Newman菌株lukE在TSB、BHI、YCP、MHB和LB培养基中不同生长阶段(对数生长早期、中期、晚期和平台期)的表达情况。以表达量较低的培养基为基础，分别加入表达量最高的培养基的不同成分，分析促进lukED表达的物质。结果显示，lukE mRNA水平在YCP培养基中生长至对数晚期时表达最高；YCP组分酵母浸出物、酪蛋白氨基酸及丙酮酸钠均能显著促进lukE转录表达，前两者效果更好。

This study aimed to explore the optimal culture conditions for transcriptional expression of leukocidin ED (lukED) of Staphylococcus aureus (S. aureus). The transcription level of lukE in S. aureus strain Newman was detected at different growth phases (early-exponential, middle-exponential, late-exponential and stationary phases) in TSB, BHI, YCP, MHB and LB media by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Based on the medium mediating the lowest transcription level of lukE, the different compositions of medium producing the highest mRNA level of lukE was added to discover the substances promoting lukED expression. The findings showed that lukE mRNA level was highest at the late-exponential phase in YCP medium, in which yeast extract, casamino acids and sodium pyruvate could all strikingly increase the expression of lukE, with the first two being better.