本实验室在1例气管切开患者的痰培养标本中，发现了1种膜状生长的革兰阴性杆菌。使用全自动细菌鉴定仪Vitek2 Compact鉴定该细菌，没有得到阳性结果。分别使用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS)和 16S 核糖体RNA(16S ribosomal RNA，16S rRNA)测序的方法对该菌进行鉴定，结果显示为肢体凯斯特菌。该菌是一种革兰氏阴性非发酵菌。回顾国外文献仅有少量病例报道，该菌可能为条件致病菌，可在气管切开患者的痰液、肺泡灌洗液以及经皮造瘘患者的尿液中分离，还可引起菌血症并参与慢性耳炎的发生发展。国内文献对肢体凯斯特菌的分离研究罕见报道。本实验室将此病例及该菌的基本特性报道如下，以加强临床微生物检验人员对其的认识和重视。

In the sputum culture of a patient with tracheotomy, we found a kind of Gram-negative bacteria with membrane growth, which was not identified by Vitek2 Compact. Then, the strain was identified by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and 16S ribosomal RNA (16S rRNA) sequencing, and the results showed that the strain was Kerstersia gyiorum. Kerstersia gyiorum is a kind of Gram-negative non-fermenting bacterium. A few of foreign cases reported that this bacterium might be a conditional pathogen, which could be isolated from sputum and alveolar lavage fluid of tracheotomy patients, and from urine of percutaneous fistula patients. This bacterium could also cause bacteremia and participate in the occurrence and development of chronic otitis media. Chinese literature on the isolation of Kerstersia gyiorum is rare. The strain of the bacterium was isolated, and the basic characteristics of the bacterium were reported as follows to enable clinical microbial laboratory personnel to strengthen the understanding and attention on it.