目的 克隆HHV-6特异性CD4⁺ T细胞，并分析其基本特征。方法 微孔有限稀释法克隆HHV-6特异性CD4⁺ T细胞；3H-TdR掺入法细胞增殖试验筛选HHV-6 特异性CD4+ T细胞克隆；FACS分析HHV-6特异性CD4⁺ T细胞克隆的表面标志；ELISA检测HHV-6特异性CD4⁺ T细胞克隆的细胞因子分泌水平。结果 获得的细胞克隆中有4株以HHV-6感染细胞裂解物特异的方式增殖，并且其增殖水平与HHV-6感染细胞裂解物呈剂量依赖性；细胞克隆表面标志为CD3⁺CD4⁺；W-2和W-4细胞克隆的细胞因子分泌以IL-10为主，W-1细胞克隆的细胞因子分泌以IL-10及IFN-γ为主，W-3细胞克隆的细胞因子分泌以IFN-γ为主。结论 初步建立HHV-6特异性CD4⁺ T细胞克隆，并分析其表面标志和细胞因子分泌水平，为HHV-6特异性Treg细胞的克隆、筛选及其功能的研究奠定基础。

Objective To establish the HHV-6-specific CD4⁺ T cell clones and further study the character of HHV-6-specific CD4⁺ T cells. Methods We acquired CD4⁺ T cell clones by the liquid microwell limiting dilution technique, and selected HHV-6-specific CD4⁺ T cell clones by means of 3H-thymidine uptake. The phenotype of HHV-6-specific T cell clones were analyzed with FACS. We tested the IL-10, IL-4 or IFN-γ secretion from HHV-6-specific CD4⁺ T cell clones by ELISA. Results Of the five HHV-6-specific CD4⁺ T cell clones, four showed proliferative responses to the stimulation with HHV-6 infected JJHAN lysates. Moreover, proliferation responses of HHV-6-specific CD4⁺T cell clones depended on the concentration of the HHV-6-infected JJHAN lysates. The phenotypic analysis with FACS demonstrated that HHV-6-specific T cell clones consisted of CD3⁺ CD4⁺ T cells. CD4⁺ T cell clones-W-2 and W-4 possessed the feature of high levels of IL-10 production, while CD4⁺ T cell clones-W-1 possessed the feature of high levels of IL-10 and IFN-γ production and W-3 possessed the feature of high levels of IFN-γ production. Conclusions The HHV-6-specific CD4⁺ T cell clones, were established in our study so as to further study their pneotype, the character of cytokine secrection, and to provide experimental basis for selecting HHV-6-specific CD4⁺ Treg cell clones.