1. Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China; 2. University of Chinese Academy of Sciences, Beijing 100039, China; 3. University of Hubei, Wuhan 430062, China
Abstract：Ebola virus belongs to filoviridae. Zaire Ebola virus has a high mortality rate up to 90%. It is transmitted through blood, secretions, organs or other body fluids of infected people or animals. Unfortunately, there are still no approved drugs and vaccines for Ebola virus infection. Considering the increasing commercial and personal communication with countries in Africa, the possibility of Ebola virus invading into China by infected animals and human highlights the necessity for the development of effective vaccines. In this study, a series of glycoproteins (GPs) were expressed by using pET28a(＋) and pcDNA3.1(＋) in prokaryotic and eukaryotic cells, respectively. The results showed that GP1(33313 aa), GP1(190313 aa) and nonstructural secretory glycoprotein (sGP) were expressed in Escherichiacoli BL21(DE3)in inclusion bodies, while no GP2(502632 aa) expression could be detected under the same condition. The edited GP, GP1 and GP2 were expressed in HEK293T cells, but not in BHK21 cells. The data provide some clues for the development of antiviral serum and effective drugs for the treatment of Ebola virus infection.
