Construction and application of alphavirus single round particle
SUN Xinran, TIAN Sicheng, MA Yanlong, FENG Fei, ZHANG Rong
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
Abstract：Alphaviruses mainly include old-world alphaviruses that cause arthritis and new-world alphaviruses that cause fatal encephalitis, which can lead to serious diseases in humans. In order to establish a safe and convenient tool to study the entry mechanism of alphaviruses, this study established pseudovirus particle system-based on murine leukemia virus (MLV) and virus replicon particle (VRP) system-based on Sindbis viurs (SINV). Using these pseudoviruses and VRPs, the effects of knockout of heparan sulfate biosynthesis related gene B3 gat3 (beta-1, 3-glucuronyltransferase 3) and arthritogenic alphavirus related entry receptor gene Mxra8 (matrix remodeling associated 8) on cell entry of different alphavirus members were verified. Therefore, the MLV-based pseudovirus and SINV-based VRP systems in this study provide a safe and reliable experimental tool for the research on the entry mechanism of alphavirus members and the development of antiviral strategy.
孙欣然，田思成，马艳龙，冯飞，张荣. 甲病毒单轮感染颗粒的构建及其应用[J]. 微生物与感染, 2023, 18(3): 129-136.
SUN Xinran, TIAN Sicheng, MA Yanlong, FENG Fei, ZHANG Rong. Construction and application of alphavirus single round particle. JOURNAL OF MICROBES AND INFECTIONS, 2023, 18(3): 129-136.
2023, Vol. 18 
