One case of sepsis caused by foodborne infection with Herbaspirillum huttiense
XU Maosuo1, SHEN Chunmei2, ZHOU Cong1, ZHANG Hui1
1. Department of Laboratory Medicine, Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China; 2. Department of Nosocomial Infection Prevention and Control，Shanghai Fifth People’s Hospital, Fudan University, Shanghai 200240, China
Abstract：In order to explore the ability of routine laboratory identification methods to identify clinical isolates of Herbaspirillum huttiense (H. huttiense), the source of infection of H. huttiense in the blood of this patient was analyzed. Automated biochemical identification system, matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF MS), 16S rRNA gene sequencing and rpoB gene sequencing were used to identify H. huttiense, and the specimens from multiple parts of the patient were detected. The results showed that the automatic biochemical identification system misidentified H. huttiense as Burkholderia cepacia. MALDI-TOF MS and 16S rRNA gene sequencing were not able to distinguish H. huttiense from Herbaspirillum aquaticum and Herbaspirillum camelliae, while rpoB gene sequencing could accurately identify H. huttiense. In addition to the blood, only a small amount of H. huttiense was detected in the patient’s feces. The results of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) showed the same DNA band patterns of H. huttiense from blood and feces. MALDI-TOF MS main spectra projection(MSP)cluster analysis showed that they were located at the same node, and the distance level was under 50, with the same resistance phenotype. The blood and fecal origins of H. huttiense were highly homologous. This study demonstrated the performance of automated biochemical identification system, MALDI-TOF MS, 16S rRNA gene sequencing and rpoB gene sequencing for identification of H. huttiense, which confirmed that H. huttiense could infect gastrointestinal tract through contaminated food and cause sepsis in patients with postoperative chemotherapy for rectal adenocarcinoma.
徐茂锁1，申春梅2，周聪1，张慧1. 哈特草螺菌食源性感染致脓毒症1例[J]. 微生物与感染, 2023, 18(5): 284-289.
XU Maosuo1, SHEN Chunmei2, ZHOU Cong1, ZHANG Hui1. One case of sepsis caused by foodborne infection with Herbaspirillum huttiense. JOURNAL OF MICROBES AND INFECTIONS, 2023, 18(5): 284-289.
2023, Vol. 18 
