Establishment of an animal model for biofilm infection of Staphylococcus epidermidis
LIU Hua-Yong1, WU You-Cong1,2, HE Nian-An3, HU Jian1, XU Tao1, GONG Ting1, HAN Hai-Yang1, WU Yang1, QU Di1
1. Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Integrated Laboratory of Pathogenic Biology, School of Preclinical Medicine, Dali University, Dali 671000, China; 3. Anhui Medical University Provincial Hospital, Hefei 230001, China
Abstract:Staphylococcus epidermidis (S. epidermidis) is one of the common opportunistic pathogens causing nosocomial infections. Although it is known that the bacteria can form biofilms by adhering to indwelling medical devices there is no optimal animal model for in ν1νo study. To establish an animal model for biofilm infection , the serum samples from several animal species were collected and their inhibitory effects on in vitroS. epidermidis (ATCC RP62A) biofilm formation were observed. New Zealand white rabbit was selected as the leading candidate for its relatively weaker inhibition on biofilm formation. Dishes cut from 96-well plates were sterilized and implanted subcutaneously , followed by injection of 1 ml (109 CFU/ mJ) bacteria in situ. Biofilms formed on the dishes in the New Zealand white rabbit model after 72 h shared morphological similarity with that formed in νitro observed under confocal laser scanning microscope and scanning electron microscope. Furthermore, the effect of vancomycin against S. epidermidis biofilm fomation in viνo was investigated. The results showed that the viable bacterial cells in biofilm treated with vancomycin (8μg/ml, local injection) was significantly lower ( P< 0.01) than that in non-treatment group. The New Zealand white rabbit model for S. epidermidis biofilm formation was established. This model can be used for the observation of biofilm morphology and evaluation of effectiveness of antimicrobial treatment ln νiνo.
刘华勇1,武有聪1,2,何年安3,胡健1,许涛1,龚婷1,韩海燕1,吴旸1 ,瞿涤1. 表皮葡萄球菌生物膜感染动物模型的建立[J]. 微生物与感染
, 2013, 8(2): 72-79.
LIU Hua-Yong1, WU You-Cong1,2, HE Nian-An3, HU Jian1, XU Tao1, GONG Ting1, HAN Hai-Yang1, WU Yang1, QU Di1. Establishment of an animal model for biofilm infection of Staphylococcus epidermidis. Journal of Microbes and Infections, 2013, 8(2): 72-79.