金属β-­内酰胺酶(MBL)可广泛水解多种抗生素，对常规β-­内酰胺酶抑制剂如克拉维酸、舒巴坦等不敏感。产MBL肠杆菌科细菌一直是临床公认的多重耐药病原体。尤其是在发现新德里β-金属­内酰胺酶后，人们更加重视肠杆菌科细菌中检出MBL的情况，因为其所致感染日渐严重。因此，快速、准确检测出产MBL菌株是有效预防、控制感染发生与播散的重要环节。本文就肠杆菌科细菌MBL的发现及分类、耐药基因传播、检测方法等进行简要概述。

Metallo-β-lactamase (MBL) can hydrolyze a variety of antibiotics, and is insensitive to conventional β-lactamase inhibitors, such as clavulanic acid and sulbactam. After the reporting of New Delh imetallo-β- lactamase (NDM) ,Enterobacteriaceae producing MBL have been recognized as clinical multidrug-resistant pathogens,and the infectìons caused by them in clinic are becoming increasingly serious. Rapid and accurate detection of this group of strains is an important link to prevent and control the occurrence and spread of infection effectively. The research progress on MBL produced by Enterobacteriaceae covering its discovery, classification, spreading and  detection methods is reviewed in the present paper.