Distribution of N-glycosidase genes in clinical isolates of Elizabethkingia meningoseptica
LI Meng, SUN Gui-Qin, CHEN Jing, LI Tian-Sheng, WANG Lei, Celimuge, CHEN Li
Key Laboratory of Medical Molecular Virology of Ministries of Health and Education, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
Abstract：The present paper aims to investigate the distribution of two peptide: N-glycanase (PNGase) genes in Elizabethkingiameningosepticum (EM). The genomic DNAs of 65 clinical strains isolated from 3 hospitals in Zhejiang Province were extracted and subjected to polymerase chain reaction (PCR) for 16SrRNA, PNGaseF and PNGaseF-Ⅱgenes respectively. The PCR products were sequenced and compared to the known sequences in National Center of Biotechnology Information (NCBI). The results showed that the positive rate of PNGase gene was 98.5% and the PNGaseF/PNGaseF-Ⅱgene coexistence rate was 60.0%. The positive rates of PNGaseF gene and PNGaseF-Ⅱgene were 63.1% and 95.4%, respectively (matching χ2 test, P<0.01). The results indicate that the developed PCR can be used to detect PNGase gene in EM strains and PNGase activity may be crucial for the survival.
李蒙，孙桂芹，陈菁，李天胜，王蕾，策力木格，陈力. N-糖苷酶基因在脑膜炎败血伊丽莎白金菌临床分离株中的分布[J]. 微生物与感染, 2014, 9(4): 224-229.
LI Meng, SUN Gui-Qin, CHEN Jing, LI Tian-Sheng, WANG Lei, Celimuge, CHEN Li. Distribution of N-glycosidase genes in clinical isolates of Elizabethkingia meningoseptica. JOURNAL OF MICROBES AND INFECTIONS, 2014, 9(4): 224-229.
