Abstract：A Mycobacteriummarinum random mutant library using MycoMarT7 phage system was constructed. After screening, a mutant whose transposon insertion site was in MMAR_0994 (mkl) gene was obtained. The adult zebrafish were infected with the wild-type and the mutant strains respectively and the survival rates of the zebrafish were compared. The potential impact of the mutant on host innate immune system was tested by infecting the 48-hour post-fertilization zebrafish larvae using caudal vein injection. Invivo distribution of the bacteria was monitored by following green fluorescence in the larvae. The impact of the mutant on mouse macrophage cell line was tested. In addition, the acid tolerance level was also checked. The results showed that mkl mutant displayed attenuated virulence in the zebrafish infection model. The attenuated phenotypes in the zebrafish larvae and mouse macrophage cell line were observed. The invasion rates of the mutant and the wild-type were similar. The growth of the mutant was more sensitive to low pH. These results indicated that mkl may play a functional role in the interaction with host immune system.
华亦斐，王晴岚，牛辰，高谦. mkl基因在海分枝杆菌抵抗宿主免疫中的作用[J]. 微生物与感染, 2015, 10(3): 173-179.
HUA Yi-Fei, WANG Qing-Lan, NIU Chen, GAO Qian. The potential role of Mycobacterium marinummkl gene in bacterial resistance to the host immunity. JOURNAL OF MICROBES AND INFECTIONS, 2015, 10(3): 173-179.